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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MA1-150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Androgen Receptor Monoclonal Antibody (AN1-15)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-150 detects androgen receptor from human, Rhesus monkey, rat and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors. MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining. The MA1-150 immunogen is a fusion protein corresponding to residues 331-572 of human androgen receptor. Reconstitute with PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- AN1-15
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The Cortisol Response of Male and Female Choroidal Endothelial Cells: Implications for Central Serous Chorioretinopathy.
Chlorella vulgaris or Spirulina platensis mitigate lead acetate-induced testicular oxidative stress and apoptosis with regard to androgen receptor expression in rats.
A novel immunocompetent model of metastatic prostate cancer-induced bone pain.
The Impact of Hippocampal Sex Hormones Receptors in Modulation of Depressive-Like Behavior Following Chronic Anabolic Androgenic Steroids and Exercise Protocols in Rats.
Ameliorating effects of green tea extract on cadmium induced reproductive injury in male Wistar rats with respect to androgen receptors and caspase- 3.
Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats.
Differential regulation of gonadotropin-releasing hormone secretion and gene expression by androgen: membrane versus nuclear receptor activation.
Cyclin E as a coactivator of the androgen receptor.
Expression of maspin in prostate cells is regulated by a positive ets element and a negative hormonal responsive element site recognized by androgen receptor.
Immunocytochemical detection of androgen receptor in human temporal cortex characterization and application of polyclonal androgen receptor antibodies in frozen and paraffin-embedded tissues.
Atypical androgen receptor in the human melanoma cell line IIB-MEL-J.
Human androgen receptor binding to the androgen response element of prostate specific antigen.
The androgen receptor status of neuroendocrine cells in human benign and malignant prostatic tissue.
Immunohistochemistry and biochemistry in detection of androgen, progesterone, and estrogen receptors in benign and malignant human prostatic tissue.
Evidence for the presence of androgen receptors in the synovial tissue of rheumatoid arthritis patients and healthy controls.
Autoregulation of androgen receptor expression in rodent prostate: immunohistochemical and in situ hybridization analysis.
Androgen receptor and 5 alpha-reductase activity in the ductuli efferentes and epididymis of adult rhesus macaques.
Localization and regulation of estrogen, progestin and androgen receptors in the seminal vesicle of the rhesus monkey.
Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor.
Prostate androgen receptor: immunohistological localization and mRNA characterization.
Fusion proteins containing androgen receptor sequences and their use in the production of poly- and monoclonal anti-androgen receptor antibodies.
Brinks J, van Dijk EHC, Kiełbasa SM, Mei H, van der Veen I, Peters HAB, Sips HCM, Notenboom RGE, Quax PHA, Boon CJF, Meijer OC
The Journal of clinical endocrinology and metabolism 2022 Jan 18;107(2):512-524
The Journal of clinical endocrinology and metabolism 2022 Jan 18;107(2):512-524
Chlorella vulgaris or Spirulina platensis mitigate lead acetate-induced testicular oxidative stress and apoptosis with regard to androgen receptor expression in rats.
Ibrahim IA, Shalaby AA, Abd Elaziz RT, Bahr HI
Environmental science and pollution research international 2021 Aug;28(29):39126-39138
Environmental science and pollution research international 2021 Aug;28(29):39126-39138
A novel immunocompetent model of metastatic prostate cancer-induced bone pain.
Liu Z, Murphy SF, Huang J, Zhao L, Hall CC, Schaeffer AJ, Schaeffer EM, Thumbikat P
The Prostate 2020 Jul;80(10):782-794
The Prostate 2020 Jul;80(10):782-794
The Impact of Hippocampal Sex Hormones Receptors in Modulation of Depressive-Like Behavior Following Chronic Anabolic Androgenic Steroids and Exercise Protocols in Rats.
Selakovic D, Joksimovic J, Jovicic N, Mitrovic S, Mihailovic V, Katanic J, Milovanovic D, Pantovic S, Mijailovic N, Rosic G
Frontiers in behavioral neuroscience 2019;13:19
Frontiers in behavioral neuroscience 2019;13:19
Ameliorating effects of green tea extract on cadmium induced reproductive injury in male Wistar rats with respect to androgen receptors and caspase- 3.
Abdelrazek HM, Helmy SA, Elsayed DH, Ebaid HM, Mohamed RM
Reproductive biology 2016 Dec;16(4):300-308
Reproductive biology 2016 Dec;16(4):300-308
Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats.
Elgawish RAR, Abdelrazek HMA
Toxicology reports 2014;1:795-801
Toxicology reports 2014;1:795-801
Differential regulation of gonadotropin-releasing hormone secretion and gene expression by androgen: membrane versus nuclear receptor activation.
Shakil T, Hoque AN, Husain M, Belsham DD
Molecular endocrinology (Baltimore, Md.) 2002 Nov;16(11):2592-602
Molecular endocrinology (Baltimore, Md.) 2002 Nov;16(11):2592-602
Cyclin E as a coactivator of the androgen receptor.
Yamamoto A, Hashimoto Y, Kohri K, Ogata E, Kato S, Ikeda K, Nakanishi M
The Journal of cell biology 2000 Aug 21;150(4):873-80
The Journal of cell biology 2000 Aug 21;150(4):873-80
Expression of maspin in prostate cells is regulated by a positive ets element and a negative hormonal responsive element site recognized by androgen receptor.
Zhang M, Magit D, Sager R
Proceedings of the National Academy of Sciences of the United States of America 1997 May 27;94(11):5673-8
Proceedings of the National Academy of Sciences of the United States of America 1997 May 27;94(11):5673-8
Immunocytochemical detection of androgen receptor in human temporal cortex characterization and application of polyclonal androgen receptor antibodies in frozen and paraffin-embedded tissues.
Puy L, MacLusky NJ, Becker L, Karsan N, Trachtenberg J, Brown TJ
The Journal of steroid biochemistry and molecular biology 1995 Nov;55(2):197-209
The Journal of steroid biochemistry and molecular biology 1995 Nov;55(2):197-209
Atypical androgen receptor in the human melanoma cell line IIB-MEL-J.
Morvillo V, Luthy IA, Bravo AI, Capurro MI, Donaldson M, Quintans C, Calandra RS, Mordoh J
Pigment cell research 1995 Jun;8(3):135-41
Pigment cell research 1995 Jun;8(3):135-41
Human androgen receptor binding to the androgen response element of prostate specific antigen.
Luke MC, Coffey DS
Journal of andrology 1994 Jan-Feb;15(1):41-51
Journal of andrology 1994 Jan-Feb;15(1):41-51
The androgen receptor status of neuroendocrine cells in human benign and malignant prostatic tissue.
Nakada SY, di Sant'Agnese PA, Moynes RA, Hiipakka RA, Liao S, Cockett AT, Abrahamsson PA
Cancer research 1993 May 1;53(9):1967-70
Cancer research 1993 May 1;53(9):1967-70
Immunohistochemistry and biochemistry in detection of androgen, progesterone, and estrogen receptors in benign and malignant human prostatic tissue.
Brolin J, Skoog L, Ekman P
The Prostate 1992;20(4):281-95
The Prostate 1992;20(4):281-95
Evidence for the presence of androgen receptors in the synovial tissue of rheumatoid arthritis patients and healthy controls.
Cutolo M, Accardo S, Villaggio B, Clerico P, Indiveri F, Carruba G, Fecarotta E, Castagnetta L
Arthritis and rheumatism 1992 Sep;35(9):1007-15
Arthritis and rheumatism 1992 Sep;35(9):1007-15
Autoregulation of androgen receptor expression in rodent prostate: immunohistochemical and in situ hybridization analysis.
Takeda H, Nakamoto T, Kokontis J, Chodak GW, Chang C
Biochemical and biophysical research communications 1991 May 31;177(1):488-96
Biochemical and biophysical research communications 1991 May 31;177(1):488-96
Androgen receptor and 5 alpha-reductase activity in the ductuli efferentes and epididymis of adult rhesus macaques.
Roselli CE, West NB, Brenner RM
Biology of reproduction 1991 Apr;44(4):739-45
Biology of reproduction 1991 Apr;44(4):739-45
Localization and regulation of estrogen, progestin and androgen receptors in the seminal vesicle of the rhesus monkey.
West NB, Chang CS, Liao SS, Brenner RM
The Journal of steroid biochemistry and molecular biology 1990 Sep;37(1):11-21
The Journal of steroid biochemistry and molecular biology 1990 Sep;37(1):11-21
Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor.
Takeda H, Chodak G, Mutchnik S, Nakamoto T, Chang C
The Journal of endocrinology 1990 Jul;126(1):17-25
The Journal of endocrinology 1990 Jul;126(1):17-25
Prostate androgen receptor: immunohistological localization and mRNA characterization.
Chang C, Chodak G, Sarac E, Takeda H, Liao S
Journal of steroid biochemistry 1989;34(1-6):311-3
Journal of steroid biochemistry 1989;34(1-6):311-3
Fusion proteins containing androgen receptor sequences and their use in the production of poly- and monoclonal anti-androgen receptor antibodies.
Chang CS, Whelan CT, Popovich TC, Kokontis J, Liao ST
Endocrinology 1989 Aug;125(2):1097-9
Endocrinology 1989 Aug;125(2):1097-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Androgen Receptor was performed by loading 25 µg of T47D (lane 1), LNCaP (lane 2) and NIH-3T3 (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with an Androgen Receptor monoclonal antibody (Product # MA1-150) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~110 kDa in T47D and LNCaP lysates.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunolocalization of androgen receptor in primate brain using Product # MA1-150.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Androgen Receptor showing staining in the cytoplasm of formalin-fixed, paraffin-embedded human prostate tissue (B) and magnified section (C) compared with an isotype control (A). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2). Tissues were probed with an Androgen Receptor monoclonal antibody (Product # MA1-150) for 60 minutes at a dilution of 2 µg/mL and detection was performed using an HRP-conjugated mouse anti-rat secondary antibody for 45 minutes followed by DAB staining.
