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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- Ab088758 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Androgen Receptor Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Androgen Receptor Antibody, Recombinant antibody (RR632), could be used for WB and IHC.Application:WB: 1/5000 Ð 1/10000IHC: 1/100-1/200Protein FunctionSteroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RR632
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,144 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Androgen Receptor Antibody (Ab088758) - Western Blot All lanes: Recombinant Androgen Receptor Antibody (Ab088758) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 110 kDa Observed band size: 100 kDa Exposure time: 64.5 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Androgen Receptor Antibody (Ab088758) - Western Blot All lanes: Recombinant Androgen Receptor Antibody (Ab088758) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 110 kDa Observed band size: 100 kDa Exposure time: 100.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Androgen Receptor Antibody (Ab088758) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling Androgen Receptor with Recombinant Androgen Receptor Antibody (Ab088758) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
