PA5-16750

antibody from Invitrogen Antibodies

Targeting: AR AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-16750

Provider

Invitrogen Antibodies

Product name

Anti-Androgen Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA5-16750 targets Androgen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with human, mouse, and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Estradiol alleviates acinar cell apoptosis and chronic pancreatitis in male Wistar Bonn/Kobori rats.

Nakamura S, Yamada T, Hashimoto T, Takahashi S, Sogawa M, Ohara H, Nakazawa T, Sano H, Kuno A, Joh T, Nomura T, Arakawa T, Itoh M
Pancreas 2003 Apr;26(3):e59-66

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Androgen Receptor was performed by loading 50 µg of LNCaP, MCF7, HeLa, and DU145 whole cell lysates, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock T20 (Product # 37543) for 1 hour. The membrane was probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:200 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). Images were acquired on a Thermo Scientific myECL Imager (Product # 62236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Androgen Receptor polyclonal antibody (Product # PA5-16750) was used to detect immunoprecipitated Androgen Receptor. Immunoprecipitation of Androgen Receptor was performed on LNCaP cells. Antigen-antibody complexes were formed by incubating 250 µg of LNCaP whole cell lysate with 2 µg of a biotinylated Androgen Receptor monoclonal antibody (Product # MA5-13423) overnight on a rocking platform at 4C. The immune complexes were captured on 50 µL Streptavidin Agarose (Product # 20347), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Eluted sample and 20 µg of LNCaP whole cell lysate (loading control) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with StartingBlock T20 (Product # 37543) for 1 hour. The membrane was probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:200 overnight rotating at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG (Product # 31460) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). Images were acquired on a Thermo Scientific myECL Imager (Product # 62236).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Androgen Receptor (green) in LNCaP (right panel) and negative control U2OS cells (left panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.