Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC5876P - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Androgen R/NR3C4 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Androgen R/NR3C4 in direct ELISAs.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antigen sequence
P10275
- Isotype
- IgG
- Antibody clone number
- 523339
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Influence of endocrine therapy on the ratio of androgen receptor (AR) to estrogen receptor (ER) positive circulating epithelial tumor cells (CETCs) in breast cancer.
Pizon M, Lux D, Pachmann U, Pachmann K, Schott D
Journal of translational medicine 2018 Dec 14;16(1):356
Journal of translational medicine 2018 Dec 14;16(1):356
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Androgen R/NR3C4 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 PE-conjugated Monoclonal Antibody (Catalog # IC5876P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.