Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 16214-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16214-1-AP, RRID:AB_2131201
- Product name
- KLHL22 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated KLHL22 antibody (Cat. #16214-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references UBE4B modulates BET inhibitor sensitivity via KLHL22-JAK2-PIM1 axis in hepatocellular carcinoma.
Amino acids and KLHL22 do not activate mTORC1 via DEPDC5 degradation.
The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation.
COLEC10 inhibits the stemness of hepatocellular carcinoma by suppressing the activity of β-catenin signaling.
A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3(KLHL22).
UBAP2L-dependent coupling of PLK1 localization and stability during mitosis.
XAF1 prevents hyperproduction of type I interferon upon viral infection by targeting IRF7.
Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke.
Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin ligase cascade in brain development.
A novel missense variant in CUL3 shows altered binding ability to BTB-adaptor proteins leading to diverse phenotypes of CUL3-related disorders.
KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway.
KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.
SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.
Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.
Wang L, Huan XJ, Song SS, Bao XB, Tian CQ, Miao ZH, Wang YQ
Biochemical pharmacology 2025 Jul;237:116943
Biochemical pharmacology 2025 Jul;237:116943
Amino acids and KLHL22 do not activate mTORC1 via DEPDC5 degradation.
Valenstein ML, Lalgudi PV, Kedir JF, Condon KJ, Platzek A, Freund DG, Taylor MS, Xu Y, Chivukula RR, Sabatini DM
Nature 2025 Jan;637(8045):E11-E14
Nature 2025 Jan;637(8045):E11-E14
The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation.
Yao Y, Hong S, Yoshida S, Swaroop V, Curtin B, Inoki K
Cell reports 2025 Jan 28;44(1):115101
Cell reports 2025 Jan 28;44(1):115101
COLEC10 inhibits the stemness of hepatocellular carcinoma by suppressing the activity of β-catenin signaling.
Cai MN, Chen DM, Chen XR, Gu YR, Liao CH, Xiao LX, Wang JL, Lin BL, Huang YH, Lian YF
Cellular oncology (Dordrecht, Netherlands) 2024 Oct;47(5):1897-1910
Cellular oncology (Dordrecht, Netherlands) 2024 Oct;47(5):1897-1910
A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3(KLHL22).
Wang W, Liang L, Dai Z, Zuo P, Yu S, Lu Y, Ding D, Chen H, Shan H, Jin Y, Mao Y, Yin Y
Nature communications 2024 May 6;15(1):3789
Nature communications 2024 May 6;15(1):3789
UBAP2L-dependent coupling of PLK1 localization and stability during mitosis.
Guerber L, Vuidel A, Liao Y, Kleiss C, Grandgirard E, Sumara I, Pangou E
EMBO reports 2023 Jun 5;24(6):e56241
EMBO reports 2023 Jun 5;24(6):e56241
XAF1 prevents hyperproduction of type I interferon upon viral infection by targeting IRF7.
Liu BQ, Liu RB, Li WP, Mao XT, Li YN, Huang T, Wang HL, Chen HT, Zhong JY, Yang B, Chai RJ, Cao Q, Jin J, Li YY
EMBO reports 2023 Jan 9;24(1):e55387
EMBO reports 2023 Jan 9;24(1):e55387
Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke.
Jin S, Chen X, Zheng H, Cai W, Lin X, Kong X, Ni Y, Ye J, Li X, Shen L, Guo B, Abdelrahman Z, Zhou S, Mao S, Wang Y, Yao C, Gu X, Yu B, Wang Z, Wang X
iScience 2023 Jan 20;26(1):105885
iScience 2023 Jan 20;26(1):105885
Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin ligase cascade in brain development.
Kong X, Shu X, Wang J, Liu D, Ni Y, Zhao W, Wang L, Gao Z, Chen J, Yang B, Guo X, Wang Z
Development (Cambridge, England) 2022 Dec 15;149(24)
Development (Cambridge, England) 2022 Dec 15;149(24)
A novel missense variant in CUL3 shows altered binding ability to BTB-adaptor proteins leading to diverse phenotypes of CUL3-related disorders.
Kato K, Miya F, Oka Y, Mizuno S, Saitoh S
Journal of human genetics 2021 May;66(5):491-498
Journal of human genetics 2021 May;66(5):491-498
KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway.
Liu XR, Wang W, Li HM
Neoplasma 2020 Sep;67(5):1106-1113
Neoplasma 2020 Sep;67(5):1106-1113
KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.
Chen J, Ou Y, Yang Y, Li W, Xu Y, Xie Y, Liu Y
Nature 2018 May;557(7706):585-589
Nature 2018 May;557(7706):585-589
SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.
Wen D, Wu J, Wang L, Fu Z
Cell reports 2017 Nov 21;21(8):2147-2159
Cell reports 2017 Nov 21;21(8):2147-2159
Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.
Zhuo X, Guo X, Zhang X, Jing G, Wang Y, Chen Q, Jiang Q, Liu J, Zhang C
The Journal of cell biology 2015 Aug 31;210(5):727-35
The Journal of cell biology 2015 Aug 31;210(5):727-35
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The KLHL22 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human KLHL22. This antibody recognizes human, mouse, rat antigen. The KLHL22 antibody has been validated for the following applications: ELISA, WB analysis.
