PA5-51417

antibody from Invitrogen Antibodies

Targeting: NSDHL H105e3, SDR31E1, XAP104

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51417

Provider

Invitrogen Antibodies

Product name

NSDHL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LPYAMKPIDY YTETKILQER AVLGANDPEK NFLTTAIRPH GIFGPRDPQL VPILIEAARN GKMKFVIGNG KNLVDFTFVE NVVHGHILAA EQLSRDSTLG GKAFHITNDE PIPFWTFLSR I Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 92%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NSDHL in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla). Samples were probed using a NSDHL Polyclonal Antibody (Product # PA5-51417).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NSDHL using NSDHL Polyclonal Antibody (Product # PA5-51417) (A) shows similar pattern to an independent NSDHL Polyclonal Antibody (B).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NSDHL in human cell line U-251 MG shows positivity in endoplasmic reticulum & vesicles. Samples were probed using a NSDHL Polyclonal Antibody (Product # PA5-51417).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NSDHL in human cell line A-431 using a NSDHL Polyclonal Antibody (Product # PA5-51417) shows localization to endoplasmic reticulum and lipid droplets.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NSDHL in human epididymis and lymph node tissues using NSDHL Polyclonal Antibody (Product # PA5-51417). Corresponding NSDHL RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NSDHL in human cerebral cortex, epididymis, lymph node and testis using NSDHL Polyclonal Antibody (Product # PA5-51417) (A) shows similar protein distribution across tissues to an independent NSDHL Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NSDHL in human lymph node using NSDHL Polyclonal Antibody (Product # PA5-51417) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NSDHL in human epididymis using NSDHL Polyclonal Antibody (Product # PA5-51417) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NSDHL in human cerebral cortex using NSDHL Polyclonal Antibody (Product # PA5-51417).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NSDHL in human testis using NSDHL Polyclonal Antibody (Product # PA5-51417).