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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-51417 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSDHL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPYAMKPIDY YTETKILQER AVLGANDPEK NFLTTAIRPH GIFGPRDPQL VPILIEAARN GKMKFVIGNG KNLVDFTFVE NVVHGHILAA EQLSRDSTLG GKAFHITNDE PIPFWTFLSR I
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSDHL in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla). Samples were probed using a NSDHL Polyclonal Antibody (Product # PA5-51417).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSDHL using NSDHL Polyclonal Antibody (Product # PA5-51417) (A) shows similar pattern to an independent NSDHL Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NSDHL in human cell line U-251 MG shows positivity in endoplasmic reticulum & vesicles. Samples were probed using a NSDHL Polyclonal Antibody (Product # PA5-51417).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NSDHL in human cell line A-431 using a NSDHL Polyclonal Antibody (Product # PA5-51417) shows localization to endoplasmic reticulum and lipid droplets.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSDHL in human epididymis and lymph node tissues using NSDHL Polyclonal Antibody (Product # PA5-51417). Corresponding NSDHL RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSDHL in human cerebral cortex, epididymis, lymph node and testis using NSDHL Polyclonal Antibody (Product # PA5-51417) (A) shows similar protein distribution across tissues to an independent NSDHL Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSDHL in human lymph node using NSDHL Polyclonal Antibody (Product # PA5-51417) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSDHL in human epididymis using NSDHL Polyclonal Antibody (Product # PA5-51417) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSDHL in human cerebral cortex using NSDHL Polyclonal Antibody (Product # PA5-51417).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSDHL in human testis using NSDHL Polyclonal Antibody (Product # PA5-51417).
