Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA000248 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA000248, RRID:AB_1079507
- Product name
- Anti-NSDHL
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NSDHL, Gene description: NAD(P) dependent steroid dehydrogenase-like, Alternative Gene Names: H105e3, SDR31E1, XAP104, Validated applications: ICC, IHC, WB, Uniprot ID: Q15738, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Proteomic analysis of enriched lysosomes at early phase of camptothecin-induced apoptosis in human U-937 cells.
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
Proteomic analysis of enriched lysosomes at early phase of camptothecin-induced apoptosis in human U-937 cells.
Parent N, Winstall E, Beauchemin M, Paquet C, Poirier GG, Bertrand R
Journal of proteomics 2009 Aug 20;72(6):960-73
Journal of proteomics 2009 Aug 20;72(6):960-73
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human epididymis and lymph node tissues using Anti-NSDHL antibody. Corresponding NSDHL RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol