Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-29630 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Bcl-2 Antibody
- Antibody type
- Monoclonal
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Free version of NBP2-29630. Detection of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis of Bcl-2 in human A) HeLa, B) A549 and C) MCF7 lysate using Bcl-2 antibody at 2 ug/ml.
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Epitope unmasking in EDTA (compared to Citrate buffer) is superior.