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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4024 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS4024, RRID:AB_1664132
- Product name
- Bcl 2 (phospho T56) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Threonine 56.
- Description
- Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-Bcl-2 (T56) pAb at 1:500 dilutionLane1:Hela cell lysate treated with colchicineLane2:sp2/0 cell lysate treated with colchicineLane3:PC12 cell lysate treated with colchicine
