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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- V2007 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bcl-2 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Bcl-2 antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 100/D5
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples using 100/D5 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 antibody (100/D5). Note nuclear membrane & cytoplasmic staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS staining (intracellular) of Jurkat cells using 100/D5 antibody (red) and isotype control (green).
