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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- F42666 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bcl-2 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 75-110 (BH3 binding domain) from the human protein was used as the immunogen for this Bcl-2 antibody.
- Description
- Purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from mouse C2C12, rat PC-12 cell line using Bcl-2 antibody diluted at 1:500 for each lane.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Bcl-2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IP and FACS: (Left) HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using NSJ# F42666 Bcl-2 antibody for IP and a different Bcl-2 antibody for WB. (Right) FACS testing of same transfected cells with NSJ# F42666.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human testis tissue stained with Bcl-2 antibody
