PAB2440

antibody from Abnova Corporation

Targeting: BCL2 Bcl-2, PPP1R50

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2440

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2440, RRID:AB_1572849

Product name

BCL2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of BCL2.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Protein phosphatase 2A inactivates Bcl2's antiapoptotic function by dephosphorylation and up-regulation of Bcl2-p53 binding.

Deng X, Gao F, May WS
Blood 2009 Jan 8;113(2):422-8

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Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues.

Liu J, Zhou W, Li SS, Sun Z, Lin B, Lang YY, He JY, Cao X, Yan T, Wang L, Lu J, Han YH, Cao Y, Zhang XK, Zeng JZ
Cancer research 2008 Nov 1;68(21):8871-80

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using BCL2 polyclonal antibody (Cat # PAB2440) for immunoprecipitation (IP) and a different BCL2 antibody for western blot. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) using BCL2 polyclonal antibody (Cat # PAB2440) in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.

Validation comment

Flow Cytometry