Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-1052-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Bcl-2 Antibody
- Antibody type
- Monoclonal
- Description
- Bcl-2 (B-cell lymphoma 2), is a member of Bcl-2 family of regulator proteins. These proteins contain a hydrophobic cleft that binds to BH3-only proteins and to the pro-apoptotic Bcl-2 family members Bad, Bak, and Bax to inhibit apoptosis. In the absence of this binding, the proapoptotic Bcl-2 members are recruited to the OMM (Outer Mitochondrial Membrane) at which they oligomerize and cause OMM permeabilization, releasing proapoptotic effectors such as SMAC or cytochrome-c. Bcl-2 also neutralize a group of 'sensor' proteins (such as BIM), which are triggered by cytotoxic stimuli such as chemotherapy. BCL-2 proteins therefore have a central role as guardians against apoptosis, helping cancer cells to evade cell death.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A synthetic peptide corresponding t
o amino acids 41-54 (GAAPAPGIFSSQPG
-Cys), of human Bcl-2 was used as i
mmunogen.- Isotype
- IgG
- Antibody clone number
- BC1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Bcl2. Anti- Bcl2(Clone: BC1) was used at 2 µg/ml on 293, MCF7 and Jurkat lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Bcl-2 in human kidney tissue using Bcl-2 antibody (Clone: BC1) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Bcl-2 in human Prostate tissue using Bcl-2 antibody (Clone: BC1) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Bcl-2 in human Spleen tissue using Bcl-2 antibody (Clone: BC1) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Bcl-2 in human Stomach tissue using Bcl-2 antibody (Clone: BC1) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis of Bcl-2 in Jurkat cell lines using 2 µg/10^6 cells of Anti-Bcl-2 antibody (10-1052 Abeomics) . Green represent isotype control and red represent Anti-Bcl-2 antibody (Clone: BC1). Goat anti-mouse PE conjugated secondary antibody (ABEOMICS) was used.
- Protocol
- Protocol