611901

antibody from BioLegend

Targeting: BCL2 Bcl-2, PPP1R50

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

611901

Provider

BioLegend

Proper citation

BioLegend Cat#611901, RRID:AB_2064307

Product name

Purified anti-Bcl-2

Antibody type

Polyclonal

Antigen

Peptide-KLH, N-terminal

Description

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. The Poly6119 antibody has been shown to be useful for Western blotting of the human, mouse, and rat Bcl-2 protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

50 µl

Storage

Upon receipt, store at 4°C.