MA5-13666

antibody from Invitrogen Antibodies

Targeting: BCL2 Bcl-2, PPP1R50

Provider product page for MA5-13666

Western blot

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [13]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: MA5-13666 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-Bcl-2 Monoclonal Antibody (8C8)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA5-13666 targets BCL-2 alpha in FACS, IF, IHC (P), and WB applications and shows reactivity with Human and Non-human primate samples. The MA5-13666 immunogen is a Synthetic peptide, aa 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 8C8
Vial size: 500 µL
Concentration: 0.2 mg/ml
Storage: 4° C

BCL2 protein structure - MA5-13666 shown in red.

Submitted references Estrogen Receptor-β Up-Regulates IGF1R Expression and Activity to Inhibit Apoptosis and Increase Growth of Medulloblastoma.

Cookman CJ, Belcher SM
Endocrinology 2015 Jul;156(7):2395-408

Effects of estrogen replacement therapy on apoptosis and vascular endothelial growth factor expression in ocular surface epithelial cells: An experimental study.

Ozcura F, Dündar SO, Cetin ED, Beder N, Dündar M
International journal of ophthalmology 2012;5(1):64-8

Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors.

Ozer H, Yenicesu G, Arici S, Cetin M, Tuncer E, Cetin A
Diagnostic pathology 2012 Sep 20;7:124

Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.

Fan C, Lin X, Wang E
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2012 Aug;33(4):1045-52

The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.

Demirkesen C, Tüzüner N, Esen T, Lebe B, Ozkal S
Leukemia research 2011 Sep;35(9):1269-72

Recurrence of benign meningiomas: predictive value of proliferative index, BCL2, p53, hormonal receptors and HER2 expression.

Abdelzaher E, El-Gendi SM, Yehya A, Gowil AG
British journal of neurosurgery 2011 Dec;25(6):707-13

Radiotherapy in laryngeal carcinoma: can a panel of 13 markers predict response?

Wildeman MA, Gibcus JH, Hauptmann M, Begg AC, van Velthuysen ML, Hoebers FJ, Mastik MF, Schuuring E, van der Wal JE, van den Brekel MW
The Laryngoscope 2009 Feb;119(2):316-22

Prognostic significance of cell proliferation and apoptosis-regulating proteins in Epstein-Barr virus positive and negative pediatric Hodgkin lymphoma.

Aktaş S, Kargi A, Olgun N, Diniz G, Erbay A, Vergin C
Lymphatic research and biology 2007;5(3):175-82

Curcumin-induced apoptosis in human leukemia cell HL-60 is associated with inhibition of telomerase activity.

Mukherjee Nee Chakraborty S, Ghosh U, Bhattacharyya NP, Bhattacharya RK, Dey S, Roy M
Molecular and cellular biochemistry 2007 Mar;297(1-2):31-9

Incidental calcifying fibrous tumor of the stomach presenting as a polyp.

Elpek GO, Küpesiz GY, Ogüs M
Pathology international 2006 Apr;56(4):227-31

Tea-induced apoptosis in human leukemia K562 cells as assessed by comet formation.

Chakraborty S, Kundu T, Dey S, Bhattacharya RK, Siddiqi M, Roy M
Asian Pacific journal of cancer prevention : APJCP 2006 Apr-Jun;7(2):201-7

Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin.

Kundu T, Dey S, Roy M, Siddiqi M, Bhattacharya RK
Cancer letters 2005 Dec 8;230(1):111-21

Prognostic factors in transitional cell cancer of the bladder: an emerging role for Bcl-2 and p53.

Ong F, Moonen LM, Gallee MP, ten Bosch C, Zerp SF, Hart AA, Bartelink H, Verheij M
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2001 Nov;61(2):169-75

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of BCL-2 alpha using BCL-2 alpha Monoclonal Antibody (Product # MA5-13666) on HeLa Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Raji (Lane 1), THP-1 (Lane 2) and HeLa (Lane 3). The blots were probed with Anti-BCL-2 Mouse Monoclonal Antibody (Product # MA5-13666, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 26 kDa band corresponding to BCL-2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of BCL-2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled BCL-2 (8C8) Mouse Monoclonal Antibody (MA513666) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 alpha antibody using peroxidase-conjugate and DAB chromogen. Note absence of staining of reactive cells in the germinal center.