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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-15668 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bcl-2 Monoclonal Antibody (8E12)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-15668 targets BCL-2 in indirect ELISA, FACS, IF, and IHC applications and shows reactivity with Human and mouse samples. The MA5-15668 immunogen is synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH. MA5-15668 detects BCL-2 which has a predicted molecular weight of approximately 26kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8E12
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Metabolic Profiling, Chemical Composition, Antioxidant Capacity, and In Vivo Hepato- and Nephroprotective Effects of Sonchus cornutus in Mice Exposed to Cisplatin.
Elhady SS, Abdelhameed RFA, Mehanna ET, Wahba AS, Elfaky MA, Koshak AE, Noor AO, Bogari HA, Malatani RT, Goda MS
Antioxidants (Basel, Switzerland) 2022 Apr 22;11(5)
Antioxidants (Basel, Switzerland) 2022 Apr 22;11(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using BCL-2 monoclonal antibody (Product # MA5-15668) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 monoclonal antibody (Product # MA5-15668) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 3T3L1 cells using BCL-2 monoclonal antibody (Product # MA5-15668) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The effect of S. cornutus crude extract on the expression levels of Bcl-2 determined by immunohistochemistry in the ( A ) liver and ( B ) kidney tissues of the experimental mice. Data of the percentage of positive stained area are expressed as mean +- SD and analyzed using one-way ANOVA followed by Bonferroni's post hoc test ( n = 8). * significantly different vs. the normal control group; # significantly different vs. the cisplatin group; ^ significantly different vs. the S. cornutus (250 mg/kg) group. Differences were considered significant at p < 0.05.
