PA5-11379

antibody from Invitrogen Antibodies

Targeting: BCL2 Bcl-2, PPP1R50

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-11379

Provider

Invitrogen Antibodies

Product name

Bcl-2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

2.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Rictor Activates Cav 1 Through the Akt Signaling Pathway to Inhibit the Apoptosis of Gastric Cancer Cells.

Cao RZ, Min L, Liu S, Tian RY, Jiang HY, Liu J, Shao LL, Cheng R, Zhu ST, Guo SL, Li P
Frontiers in oncology 2021;11:641453

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Retinal Protection from LED-Backlit Screen Lights by Short Wavelength Absorption Filters.

Sanchez-Ramos C, Bonnin-Arias C, Blázquez-Sánchez V, Aguirre-Vilacoro V, Cobo T, García-Suarez O, Perez-Carrasco MJ, Alvarez-Peregrina C, Vega JA
Cells 2021 Nov 19;10(11)

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Diazoxide inhibits of ER stress‑mediated apoptosis during oxygen‑glucose deprivation in vitro and cerebral ischemia‑reperfusion in vivo.

Lei X, Lei L, Zhang Z, Cheng Y
Molecular medicine reports 2018 Jun;17(6):8039-8046

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a Bcl-2 polyclonal antibody (Product # PA5-11379) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a Bcl-2 polyclonal antibody (Product # PA5-11379) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Bcl-2 in HeLa cells. Samples were incubated in Bcl-2 polyclonal antibody (Product # PA5-11379) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bcl-2 in paraffin-embedded Human placenta tissue. Samples were incubated with Bcl-2 polyclonal antibody (Product # PA5-11379) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bcl-2 in paraffin-embedded Human tonsil tissue. Samples were incubated with Bcl-2 polyclonal antibody (Product # PA5-11379) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Pretreatment with DZX modulates the protein expression of Bcl-2, caspase-12, caspase-3, Bax, CHOP and GRP78 in rats subjected to I/R. Western blot analysis was used to detect the protein expression levels of Bcl-2, caspase-12, caspase-3, Bax, CHOP and GRP78 in hippocampal cells isolated from I/R-treated rats. Rats were treated with DZX with or without 5-HD and subjected to cerebral ischemia for 2 h followed by 12 h of reperfusion. Sham rats were not subjected to cerebral ischemia. DZX, diazoxide; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein; CHOP, C/EBP homologous protein; GRP78, 78 kDa glucose-regulated protein; I/R, ischemia/reperfusion; 5-HD, 5-hydroxydecanoate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Immunohistochemical detection of Bcl-2 ( upper panel ), Bcl-X ( central panel ) and Caspase-3 ( lower panel ) in the three groups of rats investigated. LED: animals exposed to LED-backlit screen for 3 months; LED + F: animals exposed to filtered LED-backlit screen for 3 months. GCL: ganglionic cells layer; INL: inner nuclear layer; ONL: outer nuclear layer.