Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB56091-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CA125/MUC16 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CA125/MUC-16 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8WX17
- Isotype
- IgG
- Antibody clone number
- 986811
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CA125/MUC16 in Human Ovarian Cancer Tissue. Ovarian Cancer Antigen CA125 (CA125/MUC16) was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human CA125/MUC16 Monoclonal Antibody (Catalog # MAB56091) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CA125/MUC16 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical cancer cell line was stained with Mouse Anti-Human CA125/MUC16 Monoclonal Antibody (Catalog # MAB56091, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.