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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AB-557-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Rat CNTF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects rat CNTF in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5-10% cross-reactivity with human CNTF is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P20294- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ciliary neurotrophic factor mediates dopamine D2 receptor-induced CNS neurogenesis in adult mice.
Yang P, Arnold SA, Habas A, Hetman M, Hagg T
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Feb 27;28(9):2231-41
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Feb 27;28(9):2231-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CNTF in Rat Spinal Cord. CNTF was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Neuron Survival in Response to CNTF and Neutralization by Rat CNTF Antibody. Recombinant Rat CNTF (Catalog # 557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND50 is typically 7-15 µg/mL.
