ANT-031-50UL

antibody from Invitrogen Antibodies

Targeting: CNTF HCNTF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANT-031-50UL

Provider

Invitrogen Antibodies

Product name

CNTF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using Anti-CNTF Antibody on: - 1.Recombinant human CNTF protein (#C-240), 20 ng. 2. Recombinant human Cardiotrophin-1 protein (#C-200), 20 ng.3.Recombinant human LIF protein (#L-200), 20 ng.4. mIL-6,20 ng.5.Recombinant human CNTF protein (#C-240), 20 ng.Lanes 1-4. Anti-CNTF Antibody (#ANT-031), (1:200).Lane 5. Anti-CNTF Antibody , preincubated with CNTF Blocking Peptide (#BLP-NT031). Antibody specificity is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using Anti-CNTF Antibody on: - 1.Recombinant human CNTF protein (#C-240), 20 ng. 2. Recombinant human Cardiotrophin-1 protein (#C-200), 20 ng.3.Recombinant human LIF protein (#L-200), 20 ng.4. mIL-6,20 ng.5.Recombinant human CNTF protein (#C-240), 20 ng.Lanes 1-4. Anti-CNTF Antibody (#ANT-031), (1:200).Lane 5. Anti-CNTF Antibody , preincubated with CNTF Blocking Peptide (#BLP-NT031). Antibody specificity is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of CNTF in rat C6 glioma cells - Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF Antibody (#ANT-031), (1:200), followed by goat Anti-rabbit-DyLight-594 secondary Antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Ciliary neurotrophic factor (CNTF) in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-CNTF Antibody (#ANT-031), (1:200). A. CNTF staining (green) reveals astrocyte-profiles (arrows) in the hilus (H) of the dentate gyrus. B. Staining the same section with the astrocyte marker, Anti-glial fibrillary acidic protein (red), visualizes astrocytes in the hilus. C. Merge of the two images shows that CNTF is indeed expressed in astrocytes.