Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-257-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CNTF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CNTF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat CNTF is observed and less than 1% cross-reactivity with rhCNTF R alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P26441
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration.
The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
Hu Y, Leaver SG, Plant GW, Hendriks WT, Niclou SP, Verhaagen J, Harvey AR, Cui Q
Molecular therapy : the journal of the American Society of Gene Therapy 2005 Jun;11(6):906-15
Molecular therapy : the journal of the American Society of Gene Therapy 2005 Jun;11(6):906-15
The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
He W, Gong K, Smith DK, Ip NY
FEBS letters 2005 Aug 15;579(20):4317-23
FEBS letters 2005 Aug 15;579(20):4317-23
Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
Koh SW
Investigative ophthalmology & visual science 2002 Sep;43(9):2887-96
Investigative ophthalmology & visual science 2002 Sep;43(9):2887-96
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
He W, Gong K, Zhu G, Smith DK, Ip NY
FEBS letters 2002 Mar 13;514(2-3):214-8
FEBS letters 2002 Mar 13;514(2-3):214-8
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
He W, Gong K, Zhu G, Smith DK, Ip NY
FEBS letters 2002 Mar 13;514(2-3):214-8
FEBS letters 2002 Mar 13;514(2-3):214-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody. Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND50 is typically 3-8 µg/mL.