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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- A02352 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Calcitonin/Calca Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Calcitonin detection. Tested with IHC-P in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Different effects of implanting sensory nerve or blood vessel on the vascularization, neurotization, and osteogenesis of tissue-engineered bone in vivo.
STZ treatment induced apoptosis of retinal cells and effect of up-regulation of calcitonin gene related peptide in rats.
The integration of NSC-derived and host neural networks after rat spinal cord transection.
Fan JJ, Mu TW, Qin JJ, Bi L, Pei GX
BioMed research international 2014;2014:412570
BioMed research international 2014;2014:412570
STZ treatment induced apoptosis of retinal cells and effect of up-regulation of calcitonin gene related peptide in rats.
Yang JH, Guo Z, Zhang T, Meng XX, Sun T, Wu J
Journal of diabetes and its complications 2013 Nov-Dec;27(6):531-7
Journal of diabetes and its complications 2013 Nov-Dec;27(6):531-7
The integration of NSC-derived and host neural networks after rat spinal cord transection.
Lai BQ, Wang JM, Duan JJ, Chen YF, Gu HY, Ling EA, Wu JL, Zeng YS
Biomaterials 2013 Apr;34(12):2888-901
Biomaterials 2013 Apr;34(12):2888-901
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Calcitonin using anti-Calcitonin antibody (A02352).Calcitonin was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Calcitonin Antibody (A02352) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
