Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB1083P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IFN-alpha/beta R2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IFN-alpha/beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta , rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Mycobacterium tuberculosis Inhibits Autocrine Type I IFN Signaling to Increase Intracellular Survival.
Banks DA, Ahlbrand SE, Hughitt VK, Shah S, Mayer-Barber KD, Vogel SN, El-Sayed NM, Briken V
Journal of immunology (Baltimore, Md. : 1950) 2019 Apr 15;202(8):2348-2359
Journal of immunology (Baltimore, Md. : 1950) 2019 Apr 15;202(8):2348-2359
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IFN-alpha/beta R2 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IFN-alpha/beta R2 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1083P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.