MA5-23848

antibody from Invitrogen Antibodies

Targeting: IL10RB CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2

Western blot (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23848

Provider

Invitrogen Antibodies

Product name

IL10RB Monoclonal Antibody (90220)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-10 R alpha, rhIFN-gamma RI, and rhIFN-gamma R2 is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

90220

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of IL10RB in Human PBMC monocytes. Samples were incubated in IL10RB monoclonal antibody (Product # MA5-23848) or isotype control antibody followed by PE-conjugated Anti-Mouse IgG Secondary Antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of IL10RB in LPS-activated human peripheral blood mononuclear cells (PBMC). Samples were incubated in IL10RB monoclonal antibody (Product # MA5-23848). Recombinant Human IL‚10 inhibits IL‚1β secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‚1β/IL‚1F2 Quantikine ELISA Kit. IL‚1β secretion inhibited by Recombinant Human IL‚10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‚10 Rβ Monoclonal Antibody. The ND50 is typically 0.5-2 µg/mL in the presence of LPS (0.25 ng/mL).