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LearnPA5-112888
antibody from Invitrogen Antibodies
Targeting: IL10RB
CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-112888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL10RB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.95 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IL10RB in multiple sample types using a IL10RB polyclonal antibody (Product # PA5-112888) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Rat heart tissue, Rat liver tissue, Rat kidney tissue .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of IL10RB in MCF-7 cells using a Polyclonal antibody (Product # PA5-112888) at a dilution of 1:66. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded IL10RB in human glioma using a IL10RB Polyclonal antibody (Product # PA5-112888) at a dilution of 1:100.
