PA5-112888

antibody from Invitrogen Antibodies

Targeting: IL10RB CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-112888

Provider

Invitrogen Antibodies

Product name

IL10RB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

3.95 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IL10RB in multiple sample types using a IL10RB polyclonal antibody (Product # PA5-112888) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Rat heart tissue, Rat liver tissue, Rat kidney tissue .

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of IL10RB in MCF-7 cells using a Polyclonal antibody (Product # PA5-112888) at a dilution of 1:66. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.