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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- N1014 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TLR9 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific TLR9 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- TR9541
- Vial size
- 25 ug, 100 ug
- Concentration
- 0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
- Storage
- Aliquot the TLR9 antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples with TLR9 antibody at 2ug/ml.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse samples with TLR9 antibody at 2ug/ml.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil stained with TLR9 antibody at 5ug/ml.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human stomach tissue with TLR9 antibody at 5ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of human PBMC (lymphocytes) with TLR9 antibody at 0.5ug/10^6 cells. Green: isotype control; Red: TLR9 antibody.
