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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- 10-3003 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-TLR9 Antibody
- Antibody type
- Monoclonal
- Description
- TLR9, a member of toll-like receptor family are central to the innate immunity by identifying pathogen associated molecular patterns (PAMPs). TLR9 identify unmethylated CpG dinucleotides present in bacterial DNA leading to NF-kB activation.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant TLR9 p
rotein (amino acids 100-290) was us
ed as the immunogen for the antibod
y.- Isotype
- IgG
- Antibody clone number
- ABM1C51
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of TLR9. Anti- TLR9 antibody (Clone: ABM1C51) was used at 2 µg/ml on (1) Raji and (2) EL4 lysates.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of TLR9. Anti- TLR9 antibody (Clone: ABM1C51) was used at 4 µg/ml on Daudi lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR9 in human stomach tissue using TLR9 antibody (Clone: ABM1C51) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR9 in human Liver tissue using TLR9 antibody (Clone: ABM1C51) at 20 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR9 in human PBMC (Lymphocytes) using 0.5 µg/10^6 cells of TLR9 antibody (Clone: ABM1C51). Green represents isotype control; red represents anti-TLR9 antibody. Goat anti-mouse PE conjugate was used as secondary.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR9 in Raji cells using 0.5 µg/10^6 cells of TLR9 antibody (Clone: ABM1C51). Green represents isotype control; red represents anti-TLR9 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol
