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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- NBP1-50750 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50750, RRID:AB_11019626
- Product name
- Mouse Monoclonal Pancreatic Amylase Alpha Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pancreatic Amylase Alpha Antibody (RPA-B5) [NBP1-50750] - Pancreatic tissue lysate (lane 1) and recombinant Amylase (lane 2). The antibody detects the Rat Amylase at the expected molecular weight of 54 kDa in the pancreatic lysate and recombinant protein at 60 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Pancreatic Amylase Alpha Antibody (RPA-B5) [NBP1-50750] - Serial dilution of the antibody, starting with 2 ug/well was done to check for the affinity. 1 ug of coated antigen can be sensitively detected by anti-rat Pancreatic Amylase antibody, clone B5 used at 30 pg/well concentration.
