19194-1-AP

antibody from Proteintech Group

Targeting: WDR45 JM5, NBIA5, WDRX1, WIPI4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

19194-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#19194-1-AP, RRID:AB_2215404

Product name

WDR45 antibody

Antibody type

Polyclonal

Description

KD/KO validated WDR45 antibody (Cat. #19194-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

WDR45 deficiency shortens axon length in dopaminergic neurons from patient-derived iPSCs.

Huang N, Ouyang Q, Gong Y, Xiang J, Zhang Q, Chen C, Ding Y, An Y
Human molecular genetics 2025 Oct 14;34(21):1836-1843

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Regulation of N-degron recognin-mediated autophagy by the SARS-CoV-2 PLpro ubiquitin deconjugase.

Ayala-Torres C, Liu J, Dantuma NP, Masucci MG
Autophagy 2025 May;21(5):1019-1038

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AAV-Mediated Gene Transfer of WDR45 Corrects Neurological Deficits in the Mouse Model of Beta-Propeller Protein-Associated Neurodegeneration.

Carisi MC, Shamber C, Bishop M, Sangster M, Chandrachud U, Meyerink B, Pilaz LJ, Grishchuk Y
Human gene therapy 2025 Mar;36(5-6):637-652

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β-propeller protein-associated neurodegeneration protein WDR45 regulates stress granule disassembly via phase separation with Caprin-1.

Li Y, Fang J, Ding Y, Zhang X, Liu Y, Qiu W, Xu H, Kang Y, Chen J, Gao Y, Zhao YG, Yang P, Wang B, Tian W, Chen Y, Bi W, Zhang P
Nature communications 2025 Jun 5;16(1):5227

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The Rubicon-WIPI axis regulates exosome biogenesis during ageing.

Yanagawa K, Kuma A, Hamasaki M, Kita S, Yamamuro T, Nishino K, Nakamura S, Omori H, Kaminishi T, Oikawa S, Kato Y, Edahiro R, Kawagoe R, Taniguchi T, Tanaka Y, Shima T, Tabata K, Iwatani M, Bekku N, Hanayama R, Okada Y, Akimoto T, Kosako H, Takahashi A, Shimomura I, Sakata Y, Yoshimori T
Nature cell biology 2024 Sep;26(9):1558-1570

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Lipid droplet accumulation in Wdr45-deficient cells caused by impairment of chaperone-mediated autophagic degradation of Fasn.

Xiong Q, Sun H, Wang Y, Xu Q, Zhang Y, Xu M, Zhao Z, Li P, Wu C
Lipids in health and disease 2024 Mar 28;23(1):91

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Cardiac glycosides restore autophagy flux in an iPSC-derived neuronal model of WDR45 deficiency.

Papandreou A, Singh N, Gianfrancesco L, Budinger D, Barwick K, Agrotis A, Luft C, Shao Y, Lenaerts AS, Gregory A, Jeong SY, Hogarth P, Hayflick S, Barral S, Kriston-Vizi J, Gissen P, Kurian MA, Ketteler R
bioRxiv : the preprint server for biology 2024 Jun 17;

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Loss of WIPI4 in neurodegeneration causes autophagy-independent ferroptosis.

Zhu Y, Fujimaki M, Snape L, Lopez A, Fleming A, Rubinsztein DC
Nature cell biology 2024 Apr;26(4):542-551

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SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes.

Zheng W, Chen Q, Liu H, Zeng L, Zhou Y, Liu X, Bai Y, Zhang J, Pan Y, Shao C
Autophagy 2023 Mar;19(3):839-857

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Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegeneration.

Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N
Human molecular genetics 2023 Aug 7;32(16):2623-2637

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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.

Häusl AS, Bajaj T, Brix LM, Pöhlmann ML, Hafner K, De Angelis M, Nagler J, Dethloff F, Balsevich G, Schramm KW, Giavalisco P, Chen A, Schmidt MV, Gassen NC
Science advances 2022 Mar 11;8(10):eabi4797

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A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation.

Aring L, Choi EK, Kopera H, Lanigan T, Iwase S, Klionsky DJ, Seo YA
Journal of neurochemistry 2022 Feb;160(3):356-375

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Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.

Lee HE, Jung MK, Noh SG, Choi HB, Chae SH, Lee JH, Mun JY
International journal of molecular sciences 2021 Oct 28;22(21)

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Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration.

Lee JH, Nam SO, Kim EK, Shin JH, Oh SH, Ryu D, Lee HE, Mun JY
American journal of medical genetics. Part A 2021 Dec;185(12):3866-3871

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Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.

Chen H, Qian Y, Yu S, Xiao D, Guo X, Wang Q, Hao L, Yan K, Lu Y, Dong X, Zhou W, Wu B, Zhou S, Wang H
European journal of medical genetics 2019 Feb;62(2):149-160

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using WDR45 antibody 19194-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 19194-1-AP(WDR45 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 19194-1-AP(WDR45 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue