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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-88474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntaphilin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.889 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.
Quintanilla RA, Tapia-Monsalves C, Vergara EH, Pérez MJ, Aranguiz A
Frontiers in cellular neuroscience 2020;14:175
Frontiers in cellular neuroscience 2020;14:175
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SNPH Polyclonal antibody (Product # PA5-88474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Syntaphilin in extracts of various cell lines using Syntaphilin Polyclonal Antibody (Product # PA5-88474) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of caspase-cleaved tau on the expression of mitochondrial accessory proteins--RhoT1/T2 and syntaphilin--in immortalized cortical neurons. Panels (A-C) show representative western blot images of CN 1.4 cells transfected with GFP, GFP-T4 (normal tau), and GFP-T4C3 (truncated tau), indicating the relative RhoT1 (A') , RhoT2 (B') , and (C') syntaphilin expression levels. Also, Panels (A'-C') show quantitative analysis of the relative expression levels of RhoT1 (A') , RhoT2 (B') , and syntaphilin (C') in immortalized cells transfected with GFP and GFP-tau (s) forms. Data are mean +- SE, n = 3. Total RNA was extracted from immortalized cortical neurons transfected with GFP, GFP-T4 (full-length tau), and GFP-T4C3 (truncated tau) and mRNA expression levels of (A'') RhoT1, (B'') RhoT2, and (C'') syntaphilin were measured by RT-PCR (Jara et al., ). Data represent mean +- SE, n = 4.
