Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-0086-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD8a Monoclonal Antibody (OKT8 (OKT-8)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance. Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- OKT8 (OKT-8)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.
Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.
Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice.
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
PD-L1 expression and prognostic impact in glioblastoma.
Increased circulating IL-9-producing CD8+ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.
TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.
Autologous Graft versus Host Disease: An Emerging Complication in Patients with Multiple Myeloma.
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.
Epigenetic biomarkers of T-cells in human glioma.
TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.
Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.
The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Guo X, Jiang H, Shi B, Zhou M, Zhang H, Shi Z, Du G, Luo H, Wu X, Wang Y, Sun R, Li Z
Frontiers in pharmacology 2018;9:1118
Frontiers in pharmacology 2018;9:1118
Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.
Reber AJ, Music N, Kim JH, Gansebom S, Chen J, York I
Scientific reports 2018 Apr 17;8(1):6112
Scientific reports 2018 Apr 17;8(1):6112
Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice.
Robert R, Ang C, Sun G, Juglair L, Lim EX, Mason LJ, Payne NL, Bernard CC, Mackay CR
JCI insight 2017 Aug 3;2(15)
JCI insight 2017 Aug 3;2(15)
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
Liu Z, Han H, He X, Li S, Wu C, Yu C, Wang S
Oncology letters 2016 Mar;11(3):1829-1834
Oncology letters 2016 Mar;11(3):1829-1834
PD-L1 expression and prognostic impact in glioblastoma.
Nduom EK, Wei J, Yaghi NK, Huang N, Kong LY, Gabrusiewicz K, Ling X, Zhou S, Ivan C, Chen JQ, Burks JK, Fuller GN, Calin GA, Conrad CA, Creasy C, Ritthipichai K, Radvanyi L, Heimberger AB
Neuro-oncology 2016 Feb;18(2):195-205
Neuro-oncology 2016 Feb;18(2):195-205
Increased circulating IL-9-producing CD8+ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.
Wang W, Cheng ZS, Chen YF, Lin YH
Experimental and therapeutic medicine 2016 Dec;12(6):4055-4060
Experimental and therapeutic medicine 2016 Dec;12(6):4055-4060
TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.
Reichwald K, Jørgensen TZ, Skov S
PloS one 2014;9(8):e105627
PloS one 2014;9(8):e105627
Autologous Graft versus Host Disease: An Emerging Complication in Patients with Multiple Myeloma.
Batra A, Cottler-Fox M, Harville T, Rhodes-Clark BS, Makhoul I, Nakagawa M
Bone marrow research 2014;2014:891427
Bone marrow research 2014;2014:891427
CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.
Zhang W, Nie L, Wang Y, Wang XP, Zhao H, Dongol S, Maharjan S, Cheng L
PloS one 2013;8(6):e66286
PloS one 2013;8(6):e66286
MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.
Dössinger G, Bunse M, Bet J, Albrecht J, Paszkiewicz PJ, Weißbrich B, Schiedewitz I, Henkel L, Schiemann M, Neuenhahn M, Uckert W, Busch DH
PloS one 2013;8(4):e61384
PloS one 2013;8(4):e61384
Epigenetic biomarkers of T-cells in human glioma.
Wiencke JK, Accomando WP, Zheng S, Patoka J, Dou X, Phillips JJ, Hsuang G, Christensen BC, Houseman EA, Koestler DC, Bracci P, Wiemels JL, Wrensch M, Nelson HH, Kelsey KT
Epigenetics 2012 Dec 1;7(12):1391-402
Epigenetics 2012 Dec 1;7(12):1391-402
TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.
Arimura Y, Vang T, Tautz L, Williams S, Mustelin T
Molecular immunology 2008 Jun;45(11):3074-84
Molecular immunology 2008 Jun;45(11):3074-84
Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.
Co MD, Orphin L, Cruz J, Pazoles P, Rothman AL, Ennis FA, Terajima M
Vaccine 2008 Apr 7;26(16):1990-8
Vaccine 2008 Apr 7;26(16):1990-8
The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
Sayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, Terhorst C
Nature 1998 Oct 1;395(6701):462-9
Nature 1998 Oct 1;395(6701):462-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (open histogram) or Anti-Human CD8a PE (filled histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye