Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A02236-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD8 alpha/Cd8a Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CD8/Cd8a detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Mouse. CD8/Cd8a information: Subcellular Localization: Membrane; Single-pass type I membrane protein.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Electrospun nanofibrous mats loaded with gemcitabine and cisplatin suppress bladder tumor growth by improving the tumor immune microenvironment.
Targeting complement C5a to improve radiotherapy sensitivity in non-small cell lung cancer.
Intravoxel Incoherent Motion Diffusion-Weighted MR Imaging for Monitoring the Immune Response of Immunogenic Chemotherapy.
Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway.
A NO/ROS/RNS cascaded-releasing nano-platform for gas/PDT/PTT/immunotherapy of tumors.
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation.
USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer.
Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model.
Increased macrophage activation inhibited by tacrolimus in the kidney of diabetic rats.
Interleukin-15 gene therapy and the mammalian target of rapamycin inhibitor everolimus inhibit the growth of metastatic breast cancer.
Wang J, Yin Y, Ren X, Wang S, Zhu Y
Journal of materials science. Materials in medicine 2024 Mar 25;35(1):21
Journal of materials science. Materials in medicine 2024 Mar 25;35(1):21
Targeting complement C5a to improve radiotherapy sensitivity in non-small cell lung cancer.
Yuan M, Wang C, Wu Y, Qiao L, Deng G, Liang N, Chen F, Liu L, Chen Y, Yang Y, Wang H, Liu T, Yang X, Zhang Y, Lv Y, Suwinski R, Hu P, Zhang Y, Zhang J
Translational lung cancer research 2023 May 31;12(5):1093-1107
Translational lung cancer research 2023 May 31;12(5):1093-1107
Intravoxel Incoherent Motion Diffusion-Weighted MR Imaging for Monitoring the Immune Response of Immunogenic Chemotherapy.
Hu J, Yu X, Yin P, Du B, Cai X
Frontiers in oncology 2022;12:796936
Frontiers in oncology 2022;12:796936
Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway.
Ma Y, Yan G, Guo J, Li F, Zheng H, Wang C, Chen Y, Ye Y, Dai H, Qi Z, Zhuang G
Frontiers in immunology 2021;12:616074
Frontiers in immunology 2021;12:616074
A NO/ROS/RNS cascaded-releasing nano-platform for gas/PDT/PTT/immunotherapy of tumors.
Ding M, Shao K, Wu L, Jiang Y, Cheng B, Wang L, Shi J, Kong X
Biomaterials science 2021 Sep 7;9(17):5824-5840
Biomaterials science 2021 Sep 7;9(17):5824-5840
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation.
Ma Y, Xie B, Guo J, Chen Y, Zhong M, Lin Q, Hua J, Zhong J, Luo X, Yan G, Dai H, Qi Z
Cell transplantation 2021 Jan-Dec;30:9636897211054503
Cell transplantation 2021 Jan-Dec;30:9636897211054503
USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer.
Dai X, Lu L, Deng S, Meng J, Wan C, Huang J, Sun Y, Hu Y, Wu B, Wu G, Lovell JF, Jin H, Yang K
Theranostics 2020;10(20):9332-9347
Theranostics 2020;10(20):9332-9347
Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X
International journal of nanomedicine 2015;10:347-55
International journal of nanomedicine 2015;10:347-55
Increased macrophage activation inhibited by tacrolimus in the kidney of diabetic rats.
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W
Nephron. Experimental nephrology 2014;128(1-2):46-56
Nephron. Experimental nephrology 2014;128(1-2):46-56
Interleukin-15 gene therapy and the mammalian target of rapamycin inhibitor everolimus inhibit the growth of metastatic breast cancer.
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F
The journal of gene medicine 2013 Oct;15(10):366-74
The journal of gene medicine 2013 Oct;15(10):366-74
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). CD8 alpha was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8 alpha Antibody (A02236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image