RQ4104

antibody from NSJ Bioreagents

Targeting: CD8A CD8

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4104

Provider

NSJ Bioreagents

Product name

Cd8 alpha Antibody

Antibody type

Polyclonal

Description

This highly specific Cd8 alpha antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Cd8 alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat spleen and 2) rat thymus with Cd8a antibody at 0.5ug/ml. Expected molecular weight: 26-40 kDa depending on glycosylation level.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse spleen tissue with Cd8a antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat spleen tissue with Cd8a antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of mouse EL-4 cells with Cd8a antibody at 1ug/10^6 cells; blue=Cd8a antibody, greeen=isotype control, red=cells alone.