A02236-3

antibody from Boster Biological Technology

Targeting: CD8A CD8

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A02236-3

Provider

Boster Biological Technology

Product name

Anti-CD8 alpha/Cd8a Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for CD8 alpha/Cd8a detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse;Rat.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Electrospun nanofibrous mats loaded with gemcitabine and cisplatin suppress bladder tumor growth by improving the tumor immune microenvironment.

Wang J, Yin Y, Ren X, Wang S, Zhu Y
Journal of materials science. Materials in medicine 2024 Mar 25;35(1):21

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Targeting complement C5a to improve radiotherapy sensitivity in non-small cell lung cancer.

Yuan M, Wang C, Wu Y, Qiao L, Deng G, Liang N, Chen F, Liu L, Chen Y, Yang Y, Wang H, Liu T, Yang X, Zhang Y, Lv Y, Suwinski R, Hu P, Zhang Y, Zhang J
Translational lung cancer research 2023 May 31;12(5):1093-1107

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The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis.

Qu Y, Yang H, Li S, Li L, Li Y, Wang D
International journal of biological macromolecules 2023 May 15;237:123927

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Increased macrophage activation inhibited by tacrolimus in the kidney of diabetic rats.

Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W
Nephron. Experimental nephrology 2014;128(1-2):46-56

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Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation.

Wang Y, Wang P, Shen X, Zhao C, Zhao X, Li Y
Fertility and sterility 2007 Jun;87(6):1438-43

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CD8 alpha/Cd8a using anti-CD8 alpha/Cd8a antibody (A02236-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha/Cd8a antigen affinity purified polyclonal antibody (Catalog # A02236-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha/Cd8a at approximately 26KD. The expected band size for CD8 alpha/Cd8a is at 26KD.

Additional image