Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 301018 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#301018, RRID:AB_314136
- Product name
- LEAF™ Purified anti-human CD8a [RPA-T8]
- Antibody type
- Monoclonal
- Antigen
- CD8a
- Description
- CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Submitted references Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.
Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination.
Kmieciak M, Gowda M, Graham L, Godder K, Bear HD, Marincola FM, Manjili MH
Journal of translational medicine 2009 Oct 22;7:89
Journal of translational medicine 2009 Oct 22;7:89
Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination.
Thakral D, Dobbins J, Devine L, Kavathas PB
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7431-42
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7431-42
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with LEAF™ purified RPA-T8, followed by anti-mouse IgGs FITC
- Conjugate
- Unconjugated