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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB1806-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LILRB3/CD85a/ILT5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LILRB3/CD85a/ILT5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT4, rhILT6, rhILT7 or rhILT11 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAB68668- Isotype
- IgG
- Antibody clone number
- 222821
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Leukocyte immunoglobulin-like receptors maintain unique antigen-presenting properties of circulating myeloid dendritic cells in HIV-1-infected elite controllers.
Cytokine induced expression of programmed death ligands in human neutrophils.
Allogeneic disparities in immunoglobulin-like transcript 5 induce potent antibody responses in hematopoietic stem cell transplant recipients.
Inhibitory immunoglobulin-like receptors LILRB and PIR-B negatively regulate osteoclast development.
Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation.
Huang J, Burke PS, Cung TD, Pereyra F, Toth I, Walker BD, Borges L, Lichterfeld M, Yu XG
Journal of virology 2010 Sep;84(18):9463-71
Journal of virology 2010 Sep;84(18):9463-71
Cytokine induced expression of programmed death ligands in human neutrophils.
Bankey PE, Banerjee S, Zucchiatti A, De M, Sleem RW, Lin CF, Miller-Graziano CL, De AK
Immunology letters 2010 Apr 8;129(2):100-7
Immunology letters 2010 Apr 8;129(2):100-7
Allogeneic disparities in immunoglobulin-like transcript 5 induce potent antibody responses in hematopoietic stem cell transplant recipients.
Pfistershammer K, Lawitschka A, Klauser C, Leitner J, Weigl R, Heemskerk MH, Pickl WF, Majdic O, Böhmig GA, Fischer GF, Greinix HT, Steinberger P
Blood 2009 Sep 10;114(11):2323-32
Blood 2009 Sep 10;114(11):2323-32
Inhibitory immunoglobulin-like receptors LILRB and PIR-B negatively regulate osteoclast development.
Mori Y, Tsuji S, Inui M, Sakamoto Y, Endo S, Ito Y, Fujimura S, Koga T, Nakamura A, Takayanagi H, Itoi E, Takai T
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4742-51
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4742-51
Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation.
Masuda A, Nakamura A, Maeda T, Sakamoto Y, Takai T
The Journal of experimental medicine 2007 Apr 16;204(4):907-20
The Journal of experimental medicine 2007 Apr 16;204(4):907-20
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of LILRB3/CD85a/ILT5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB1806) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
