PA5-97926

antibody from Invitrogen Antibodies

Targeting: LILRB3 CD85a, HL9, ILT5, LIR-3, LIR3, PIR-B, PIRB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97926

Provider

Invitrogen Antibodies

Product name

LILRB3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

2.38 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of LILRB3 using a LILRB3 Polyclonal antibody (Product # PA5-97926) at a concentration of 3.9 µg/mL. Positive WB detected in: HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 70 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LILRB3 in NIH/3T3 cells using a LILRB3 polyclonal antibody (Product # PA5-97926) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.