STJ97007

antibody from St John's Laboratory

Targeting: FUT4 CD15, ELFT, FCT3A, FUC-TIV

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ97007 - Provider product page
Provider: St John's Laboratory
Product name: Anti-FUT4 antibody [Q89] (STJ97007)
Antibody type: Monoclonal
Description: Mouse monoclonal antibody anti-Alpha (1-3-Fucosyltransferase 4 is suitable for use in Immunohistochemistry, Immunofluorescence and Immunocytochemistry research applications.
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Handling: NA

FUT4 protein structure - STJ97007 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of Human-liver-cancer tissue. 1, CD15 monoclonal antibody (Q89) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200 (4Â°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200 (4Â°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200 (4Â°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using CD 15 Mouse mAb diluted at 1:500.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, CD15 monoclonal antibody (Q89) was diluted at 1:200 (4Â°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98Â°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA