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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 39558 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIRA Monoclonal Antibody (WC119.2H11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- WC119.2H11
- Vial size
- 10 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- HIRA mAb (Clone WC119.2H11) tested by Western blot. 5 µg of HIRA mAb (Clone WC15) was used to immunoprecipitate HIRA from 500 µg of colcemid-treated U2OS nuclear extract (lane 2). 5 µg of control mouse IgG was also used (lane 1). The immunoprecipitated protein was detected by Western blotting using HIRA mAb (Clone WC119.2H11) at a dilution of 5 µg/ ml.
