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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA325022 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal PACSIN2 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal PACSIN2 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PACSIN2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.4 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PACSIN2 Antibody (S357) (Cat.# TA325022) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PACSIN2 Antibody (S357) (Cat.# TA325022) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term) (Cat. #TA325022). TA325022 was diluted at 1:100 dilution. Alexa Fluor?? 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor?? 554 (red) conjugated Phalloidin (red).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
