60271-1-Ig

antibody from Proteintech Group

Targeting: MS4A1 B1, Bp35, CD20, MS4A2

Provider product page for 60271-1-Ig

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ELISA

Immunocytochemistry

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Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [48]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 60271-1-Ig - Provider product page
Provider: Proteintech Group
Product name: CD20 antibody
Antibody type: Monoclonal
Description: CD20 antibody (Cat. #60271-1-Ig) is a mouse monoclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IF, IHC, WB, ELISA.
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 4A7G3
Vial size: 20ul, 150ul

MS4A1 protein structure - 60271-1-Ig shown in red.

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CD20(+)FCRL4(+) B Cells Activate CD8(+) T Cells via MHC-I Restriction in Nasopharyngeal Carcinoma Anti-Tumor Immunity.

Zhang B, Yuan X, Gao K, You B, Wang Y, Meng L, Chen Z, Xie S, Liu Y, Dong Z, Xie S, Fan R, Wang F, Zhang J, Xie Z, Mo Y, Zhang H, Jiang W
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Single-cell profiling of tumor lineage plasticity and the immune microenvironment in transformed small cell lung cancer.

Huang J, Zhang Z, Cai G, Huang W, Chen YQ, Zhang J, Yang L, Zhou Q, Yang JJ, Wang J
Journal of translational medicine 2026 Mar 4;24(1)

Association of CCL21(+) CAFs with B-cell recruitment and TLS maturation in penile squamous cell carcinoma.

Li X, Chen D, Guo Y, Huang G, Cai Z, Xue T, Chen Z, Han H, Li Z
Cancer immunology, immunotherapy : CII 2026 Mar 17;75(4)

Single-cell spatial analysis identifies ID1-high endothelial cells in tertiary lymphoid structures as predictors of durable response to immunotherapy in non-small cell lung cancer.

Matsumoto K, Noda Y, Hachiya K, Muramatsu F, Okamoto N, Jia W, Shiroyama T, Mori M, Tamiya M, Kinehara Y, Tamiya A, Shimizu S, Suzuki H, Ueno K, Niki T, Tetsumoto S, Morimura O, Osa A, Minami T, Nojima S, Takeda Y, Shintani Y, Kumanogoh A, Takakura N
Cancer cell international 2026 Jan 6;26(1):65

Characteristics of tertiary lymphoid structures in prostate cancer and the impact of neoadjuvant therapy on their formation and maturation.

Liu S, Yu Y, Zhou J, Yang L, Yan X, Liu X, Ma K, Liu L, Wang X, Zhao C, Dong Q
Frontiers in immunology 2025;16:1663396

Machine learning-driven prediction of immune checkpoint inhibitor responses against cholangiocarcinoma: a bile biopsy perspective.

Zhang D, Li X, Wang Z, Fan J, Yang Y, Han S, Sun W, Wang D, Zhou S, Liu Z, Chen S, Yang Y, Zhu Y, Lu Z
Frontiers in immunology 2025;16:1614683

Distribution characteristics and prognostic value of TIM-1 in patients with lung adenocarcinoma.

Wen J, Yun W, Chen Y, Yin X, Cui W, Yu M, Meng X
Frontiers in immunology 2025;16:1602868

NALCN expression is down-regulated and associated with immune infiltration in gastric cancer.

Li X, Wu N, Wang C, Pei B, Ma X, Xie J, Yang W
Frontiers in immunology 2025;16:1512107

Plasma exchange and intravenous immunoglobulin prolonged the survival of a porcine kidney xenograft in a sensitized, brain-dead human recipient.

Ma S, Qi R, Han S, Li Z, Zhang X, Wang G, Liu K, Xu T, Zhang Y, Han D, Zhang J, Wei D, Fan X, Pan D, Jia Y, Li J, Wang Z, Zhang X, Yang Z, Tao K, Yang X, Dou K, Qin W
Chinese medical journal 2025 Sep 20;138(18):2293-2307

Effect of tumor draining lymph nodes in the formation and maturation of tertiary lymphoid structure in patients with lung adenocarcinoma.

Wen J, Yun W, Yin X, Meng X
BMC cancer 2025 Oct 3;25(1):1507

Analysis of Immune Cell Infiltration Distribution and Prognostic Value in Obstructive Colorectal Cancer.

Xue Y, Jiang Z, Gu J, Deng S, Cai K, Wu K
Biomedicines 2025 Oct 23;13(11)

The m(6)A demethylase FTO links TLR7 to mitochondrial oxidation driving age-associated B cell formation in systemic lupus erythematosus.

Zeng Q, Li L, Li X, Qin L, Feng T, Zhu Y, Wang J, Zou Y, Su J, Dong G, Xia W, Xu T, Zhang G, Cui Y, Lin H, Li X, Li Y
Science translational medicine 2025 Nov 5;17(823):eadu6015

MS4A1 regulates M1-polarized tumor-associated macrophage infiltration, angiogenesis, and cancer progression through the HIPPO pathway in lung adenocarcinoma.

Gao W, Wang R, Yang S, Shi Y, Cui M, Jiang R, Li R, Yu Y, Jia D, Che D
Cancer immunology, immunotherapy : CII 2025 Nov 3;74(12):356

Neoadjuvant with low-dose radiotherapy, tislelizumab, albumin-bound paclitaxel, and cisplatin for resectable locally advanced head and neck squamous cell carcinoma: phase II single-arm trial.

Liu Z, Wang D, Li G, Yi M, Zhang Z, Zhong G, Xu L, Jiang R, Zheng Y, Huang L, Peng Y, Liang L, Li J, Liu Y, Lai J, Lv X, Xu Y, Liu Q, Wang Z, Liu Z, Yang Q, Nie L, Lei J, Huang X, Liu Z, Jiang W
Nature communications 2025 May 17;16(1):4608

Prognostic implications and characterization of tumor-associated tertiary lymphoid structures genes in pancreatic cancer.

Zhang E, Ma Y, Liu Z, Zhang J, Liu W, Chen Y, Liu G, Liu X, Zhang F, Zhu Y, Yang Y, Tian X
Journal of translational medicine 2025 Mar 10;23(1):301

Ultrasound/magnetic resonance bimodal imaging-guided CD20-targeted multifunctional nanoplatform for photothermal/chemo synergistic therapy of B-cell lymphoma.

Wang Z, Huang J, Lv W, Huang C, Wang Y, Li X, Liu H, Hao L
Journal of pharmaceutical sciences 2025 Feb;114(2):967-982

C-Reactive Protein to Lymphocyte Ratio (CLR) and Lactate Dehydrogenase to Albumin Ratio (LAR) as Prognostic Biomarkers in Acral Melanoma: Association With Tertiary Lymphoid Structures and Immune Cell Infiltration.

Kang D, Su X, Wang J, Zhao L, Huang R, Zou Z
Cancer medicine 2025 Aug;14(15):e71078

Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer.

Guan X, Zhang Y, Sun R, Wang G, Bi X, Zhang Z, Zan L, Meng Y, Liu Y, Guo J, Ma J, Li J, Hu H, Zhang Y, Zhang X, Liu M, Jiang B, Liu H, Zou S, Xi Y, Zhou H, Bai W, Wu D, Wang X
Nature communications 2025 Aug 22;16(1):7834

Single-cell profiling identifies biomarkers for immunochemotherapy in esophageal squamous cell carcinoma.

Wang C, Xie G, Jia M, Xu L, Ruan R, Yu C, Kan X, Feng Y, Ying J, Huang Y, Wei W, Chen Q, Jiang Y, Hu Y, Cai L, Lu H, Song G, Shi L, Mao W, Hu J, Zhao A, Chen Q, Wang X
Cancer letters 2025 Aug 14;633:217988

Intratumoral CXCL13(+) CD160(+) CD8(+) T cells promote the formation of tertiary lymphoid structures to enhance the efficacy of immunotherapy in advanced gastric cancer.

Wang J, Liang Y, Xue A, Xiao J, Zhao X, Cao S, Li P, Dong J, Li Y, Xu Z, Yang L
Journal for immunotherapy of cancer 2024 Sep 6;12(9)

Mapping and Analysis of Protein and Gene Profile Identification of the Important Role of Transforming Growth Factor Beta in Synovial Invasion in Patients With Pigmented Villonodular Synovitis.

Li T, Xiong Y, Li J, Tang X, Zhong Y, Tang Z, Zhang Q, Luo Y
Arthritis & rheumatology (Hoboken, N.J.) 2024 Nov;76(11):1679-1695

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Bian Z, Chen B, Shi G, Yuan H, Zhou Y, Jiang B, Li L, Su H, Zhang Y
Cell death & disease 2024 Nov 11;15(11):812

CXC ligand 13 orchestrates an immunoactive microenvironment and enhances immunotherapy response in head and neck squamous cell carcinoma.

Lin X, Zhao X, Chen Y, Yang R, Dai Z, Li W, Lin C, Cao W
International journal of immunopathology and pharmacology 2024 Jan-Dec;38:3946320241227312

Pumilio1 regulates NPM3/NPM1 axis to promote PD-L1-mediated immune escape in gastric cancer.

Wang H, Zhou Z, Zhang J, Hao T, Wang P, Wu P, Su R, Yang H, Deng G, Chen S, Gu L, He Y, Zeng L, Zhang C, Yin S
Cancer letters 2024 Jan 28;581:216498

A novel lipid metabolism-based risk model associated with immunosuppressive mechanisms in diffuse large B-cell lymphoma.

Zhang Z, Zhao C, Yang S, Lu W, Shi J
Lipids in health and disease 2024 Jan 22;23(1):20

Biomarkers of Pathologic Complete Response to Neoadjuvant Immunotherapy in Mismatch Repair-Deficient Colorectal Cancer.

Li J, Hu H, Qin G, Bai F, Wu X, Ke H, Zhang J, Xie Y, Wu Z, Fu Y, Zheng H, Gong L, Xie Z, Deng Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2024 Jan 17;30(2):368-378

Tumoral C2 Regulates the Tumor Microenvironment by Increasing the Ratio of M1/M2 Macrophages and Tertiary Lymphoid Structures to Improve Prognosis in Melanoma.

Zhang G, Li S, Xiao W, Zhang C, Li T, Liao Z, Liu H, Xing R, Yao W, Yang J
Cancers 2024 Feb 23;16(5)

Spatial patterns and MRI-based radiomic prediction of high peritumoral tertiary lymphoid structure density in hepatocellular carcinoma: a multicenter study.

Long S, Li M, Chen J, Zhong L, Abudulimu A, Zhou L, Liu W, Pan D, Dai G, Fu K, Chen X, Pei Y, Li W
Journal for immunotherapy of cancer 2024 Dec 15;12(12)

Single-Cell RNA-Sequencing Reveals Peripheral T Helper Cells Promoting the Development of IgG4-Related Disease by Enhancing B Cell Activation and Differentiation.

Ji Z, Lu W, Wu S, Zhang Y, Meng D, Zhang X, Dai X, Chen H, Ma L, Sun Y, Jiang L, Kong X
International journal of molecular sciences 2023 Sep 6;24(18)

Changes in Bacteroides and the microbiota in patients with obstructed colorectal cancer: retrospective cohort study.

Cao Y, Shang F, Jin M, Deng S, Gu J, Mao F, Qin L, Wang J, Xue Y, Jiang Z, Cheng D, Liu L, Nie X, Liu T, Liu H, Cai K
BJS open 2023 Nov 1;7(6)

Remodeling of the immune and stromal cell compartment by PD-1 blockade in mismatch repair-deficient colorectal cancer.

Li J, Wu C, Hu H, Qin G, Wu X, Bai F, Zhang J, Cai Y, Huang Y, Wang C, Yang J, Luan Y, Jiang Z, Ling J, Wu Z, Chen Y, Xie Z, Deng Y
Cancer cell 2023 Jun 12;41(6):1152-1169.e7

Levels of systemic inflammation response index are correlated with tumor-associated bacteria in colorectal cancer.

Cao Y, Zheng X, Hu Y, Li J, Huang B, Zhao N, Liu T, Cai K, Tian S
Cell death & disease 2023 Jan 30;14(1):69

Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy.

Wang Q, Sun K, Liu R, Song Y, Lv Y, Bi P, Yang F, Li S, Zhao J, Li X, Chen D, Mei J, Yang R, Chen K, Liu D, Tang S
Clinical and translational medicine 2023 Aug;13(8):e1346

Lin K, Zhou Y, Lin Y, Feng Y, Chen Y, Cai L
Biomolecules 2023 Apr 9;13(4)

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Liu X, Tan S, Liu H, Jiang J, Wang X, Li L, Wu B
Hepatology (Baltimore, Md.) 2023 Apr 1;77(4):1181-1197

Integrating Bulk Transcriptome and Single-Cell RNA Sequencing Data Reveals the Landscape of the Immune Microenvironment in Thoracic Aortic Aneurysms.

Wang Q, Guo X, Huo B, Feng X, Fang ZM, Jiang DS, Wei X
Frontiers in cardiovascular medicine 2022;9:846421

RNA-Binding Protein IGF2BP1 Associated With Prognosis and Immunotherapy Response in Lung Adenocarcinoma.

Liu J, Li Z, Cheang I, Li J, Zhou C
Frontiers in genetics 2022;13:777399

Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.

Qi Z, Duan L, Yuan G, Liu J, Li J, Li G, Yu Y, Xu Y, Ma S, Pan Y, Zhang Y
Frontiers in oncology 2022;12:769895

Impaired CD8(+) T cells in term pregnancy decidua with chronic hepatitis B virus infection.

Ma Y, Liu X, Lou H, Chen L, He W, Pan Q, Liao D, Li J, Wu J, Xiong R, Liu S, Luo M, Wu F, Chen J, Liu Z, Tang L, Li Y, Gao Y
American journal of reproductive immunology (New York, N.Y. : 1989) 2022 Oct;88(4):e13610

Tertiary lymphoid structures: Associated multiple immune cells and analysis their formation in hepatocellular carcinoma.

Nie Y, Fan H, Li J, Lei X, Zhang T, Wang Y, Mao Z, Tao K, Song W
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Nov;36(11):e22586

Peritumoral TIGIT(+)CD20(+) B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer.

Liu H, Wu J, Xu X, Wang H, Zhang C, Yin S, He Y
International immunopharmacology 2022 Jul;108:108735

Fibroblast Activation Protein and Tertiary Lymphoid Structure in Colorectal Cancer Recurrence.

Hayase S, Miyoshi N, Fujino S, Yasui M, Ohue M, Minami S, Kato S, Nagae A, Sekido Y, Hata T, Hamabe A, Ogino T, Takahashi H, Uemura M, Yamamoto H, Doki Y, Eguchi H
Anticancer research 2022 Dec;42(12):5897-5907

The Combination of Jiedu Xiaoluo Decoction with Autologous Peripheral Blood Stem Cell Transplantation (APBSCT) Accelerates Disease Remission of Non-Hodgkin Lymphoma.

Zhang Y, Xiang J, Zhu N, Ge H, Sheng X, Deng S, Chen J, Yu L, Zhou Y, Shen J
Evidence-based complementary and alternative medicine : eCAM 2021;2021:2745705

Altered peripheral helper T cells in peripheral blood and muscle tissue of the patients with dermatomyositis.

Hou X, Yang C, Lin M, Tian B, Zhao S, Liu X, Yang P
Clinical and experimental medicine 2021 Nov;21(4):655-661

The distinctive characteristics of the micro-vasculature and immune cell infiltration in cystic pancreatic neuroendocrine tumors.

Gao H, Wang W, Zhang W, Xu H, Wu C, Li H, Ni Q, Yu X, Liu L
Journal of endocrinological investigation 2021 May;44(5):1011-1019

The downregulation of NCXs is positively correlated with the prognosis of stage II-IV colon cancer.

Xia Z, Wang C, Zhang H
World journal of surgical oncology 2021 Jun 14;19(1):177

Early Pattern of Epstein-Barr Virus Infection in Gastric Epithelial Cells by "Cell-in-cell".

Yue W, Zhu M, Zuo L, Xin S, Zhang J, Liu L, Li S, Dang W, Zhang S, Xie Y, Zhu F, Lu J
Virologica Sinica 2019 Jun;34(3):253-261

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Raji cells were subjected to SDS PAGE followed by western blot with 60271-1-Ig(MS4A1,CD20 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CD20 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human CD20. This antibody recognizes human,mouse antigen. The CD20 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 60271-1-Ig.pdf

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