Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 302354 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#302354, RRID:AB_2565619
- Product name
- Alexa Fluor® 594 anti-human CD20
- Antibody type
- Monoclonal
- Antigen
- Human tonsillar B cells
- Description
- CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 2H7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) then blocked with 5% FBS. The cells were then stained with 10 µg/ml CD20 (clone 2H7) Alexa Fluor® 594 (red), CD3 (clone UCHT1) Alexa Fluor® 647 (yellow), and CD14 (clone HCD14) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
- Conjugate
- Yellow dye