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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- TA591088 - Provider product page
- Provider
- OriGene
- Product name
- CD20 Mouse Monoclonal Antibody, clone 743X65
- Antibody type
- Monoclonal
- Description
- CD20 Mouse Monoclonal Antibody, clone 743X65
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MS4A1
- Isotype
- IgG
- Antibody clone number
- 743X65
- Vial size
- 100 µl
- Concentration
- 0.25mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometry on CD20 transfected HEK283F cells. Red filled graph is staining with the CD20 antibody, the unfilled pink graph is a cell transfected with an unrelated gene.
- Validation comment
- FC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Direct cell killing on Daudi & Raji cells using CD20 antibody (TA591088, clone 743X65).
- Validation comment
- Assay
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Epitopes were defined by homologous scanning mutagenesis using human-mouse CD20 chimeras. Note: this method defines the key contact points (green) and the complete set of contacts will likely be broader.
- Validation comment
- Assay
