13-0209-82

antibody from Invitrogen Antibodies

Targeting: MS4A1 B1, Bp35, CD20, MS4A2

Provider product page for 13-0209-82

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [14]
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Validations
Flow cytometry [1]
Other assay [14]

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Product number: 13-0209-82 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD20 Monoclonal Antibody (2H7), Biotin, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.
Conjugate: Biotin
Antibody clone number: 2H7
Concentration: 0.5 mg/mL

MS4A1 protein structure - 13-0209-82 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 7 Increased expression of IGF-1, FGFR-3, its ligand FGF-2 and CD20 in tumor tissue obtained on treatment with BRAF and MEK inhibitors. a , b Tumor tissue from melanoma patients ( n = 20) after kinase inhibitor therapies ( red bars ) show increased transcript levels of IGF-1, FGFR-3, CD20, and FGF-2 b when compared with same patients' pre-treatment tumor tissues ( black bars ). mRNA transcripts were determined by real time qPCR (as described in Fig. 1 legend) with levels indicated as RQ values normalized to an endogenous control (GAPDH) and relative to pre-treatment cDNA samples. c Transcript levels of IGF-1, FGFR-3, and CD20 of melanoma patients' cDNA samples on treatment showed a correlation with each other (IGF-1 and FGFR-3 (Spearman's r = 0.6936; p = 0.0376); IGF-1 and CD20 (Spearman's r = 0.5074; p = 0.0020)). d Increased presence of CD20 + B cells co-stained with IGF-1 in tumor sections obtained from patients undergoing treatment with kinase inhibitors. Representative immunostaining of a patient's tumor section pre- ( left top panel ) and on-therapy ( bottom left panel ) with BRAFi/MEKi showing co-staining of IGF-1 ( red ) and CD20 ( dark brown ). Magnified view of the co-staining is shown on top right panel and multi-spectral analysis confirming the co-localization of IGF-1 and CD20+ B cells is shown in bottom right panel ( yellow ). Scale bars : 100 mum. e Increased RNA expression of FGFR-3 in 8/21 progression biopsies ( green frame ) obta
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 8 Clinical activity of CD20 immunotargeting in metastatic melanoma patients. PET-CT scans from two different patients obtained pre- vs. post-anti-CD20 antibody therapy. a , b Note complete disappearance of a metabolically active metastatic site ( white arrow ); c , d mixed response with almost complete disappearance of one metabolically active metastatic site ( white arrow in d ) and simultaneous increase in size and metabolic activity of the other ( red arrow in d ). e Representative combined PAX5 (nuclear; purple )/CD20 (membrane; yellow ) immunofluoresence staining of patient-matched melanoma samples before and after therapy with anti-CD20 antibody (overviews ( top rows ; scale bars : 100 mum) and corresponding close ups ( bottom rows ; scale bars : 10 mum)). Note depletion of TAB cells in post-therapy tumors. CSPG staining of melanoma cells ( green ) and nuclear DAPI staining ( blue ). Right panel : double positive PAX5 (nuclear)/CD20 (membrane) immunofluoresent TAB cells
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Cell sorting of precursor B cells subsets from CD10 positively selected cells. Immunomagnetic selection and subsequent FACS were used to isolate the five populations from pediatric and adult human BM. Shown are the FACS dot plots with sorting gates to obtain CD34 + CD19 - ProB cells, CD34 + CD19 + PreBI cells, CD34 - CD19 + CD20 dim PreBII large cells, CD34 - CD19 + CD20 - PreBII small cells, and CD34 - CD19 + CD20 high IgM + Immature B cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig 5 Immunohistochemistry for Bcl-xL in the RA, LMAN, HVC and Area X of the Bengalese finch (visualized by nickel intensified DAB). A1-D4: Labeled cells in the RA at post-hatching day (P) 35 (A1, A2) and P45 (A3-A4), in the LMAN at P15 (B1, B2) and P45 (B3, B4), in the HVC at P45 (C1, C2) and in the adult (C3, C4), and in Area X at P15 (D1, D2) and P45 (D3, D4). E-H: Comparison of the densities of Bcl-xL-positive cells in the RA (E), LMAN (F), HVC (G) and Area X (H) between males and females. The borders of the song nuclei (dashed lines) were determined with the help of another set of Nissl-stained sections. The Nissl-defined border of the female Area X was difficult to clearly identify, and the dashed lines in D2 and D4 indicate the approximate region corresponding to the male Area X. Dorsal is up and caudal is right. Scale bar = 200 mum in A1-C4 and 300 mum in D1-D4. The data are expressed as the mean +- SEM. * P < 0.05, ** P < 0.01.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 4 IGF-1-dependent induction of cancer stem cell markers CD20, CD133, and CD271 (NGFR) on melanoma cells. a Melanoma cells (WM3749) co-cultured with TAB cells (days 3 and 9) show high expression of CD20 ( middle and right panels ) compared with the control culture ( left panel ) as determined by FACS analysis. Melanoma cells were co-stained with anti-CD146 (MCAM, PE-conjugated) and anti-CD20 (FITC-conjugated) antibodies to distinguish them from B cells, which are CD146-negative;percentages indicate co-expression of both markers on the malignant cells. b Melanoma cells (WM3749) co-cultured with TAB cells (day 6) show high expression of CD20, CD133 and CD271 ( left panel ) compared with minimal or low expression of those markers when tumor cells are co-cultured with NB cells ( right panel ). Co-culture of melanoma cells with TAB cells did not modulate the expression of CD144 (vascular-endothelial cadherin marker) that are normally expressed by aggressive melanomas (data not shown). Induction of CD20, CD133, and CD271 was blocked when anti-IGF-1 neutralizing antibody (10 mug/ml) was used in the co-culture ( middle panel ). Anti-IL-1, anti-PDGF or anti-VEGF antibodies had no effect on CD marker expression (data not shown). Percentages indicate co-expression of CD20, CD133, or CD271on CD146 + melanoma cells. Results are representative of two independent experiments. c Melanoma cells(WM3749, WM989 and 451Lu) cultured in the presence of recombinant IGF-1 (25 ng/ml) for 5 days sh
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 2 Long-term expansion of human germinal center B cells ex vivo. a Primary human GC B cells were transduced with the indicated oncogenes and oncogene combinations and cultured separately for up to 120 days. Graph shows calculated theoretical absolute cell numbers (+-s.e.m., n = 3). Viable cells were assessed by trypan blue exclusion. Source data are provided as a Source Data file. b Primary human GC B cells were transduced with different oncogenes and oncogene combinations and monitored by flow cytometry. Graph shows the change in cell viability assessed by scatter characteristic by flow cytometry ( +- s.e.m., n = 3). Source data are provided as a Source Data file. c Primary human GC B cells were transduced with BCL2 in combination with other transcription factors in a pooled, competitive culture. Graph shows relative abundance of transcription factors or their mutant versions over four different timepoints ( n = 3). d Primary human GC B cells were transduced with the oncogenic cocktail BCL2 and BCL6 and cultured to day 73. Representative flow cytometry analysis ( n = 3) for the expression of the GC B cell markers CD38, CD20, CD19, CD80, CD22, CD95, CXCR4, and CD86. Red histograms show GC B cells compared to primary human naive B cells (blue). e Heat map of gene expression of freshly isolated GC B cells ( n = 3), transduced GC B cells ( BCL2-BCL6, BCL2-MYC ) cultured ex vivo for 5 or 73 days ( n = 3), plasma cell line ( n = 1), naive B cells ( n = 1), and lymphoma cell li
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 IL-33 + cells in Duodenal Mucosae of active celiac patients. Representative images of immunofluorescence analysis of duodenal sections of active CD patients. IL-33 (green), nuclei (blue). Other markers in red. In some images the epithelial compartment was delimited with white dashed lines. (A). CD31 + endothelial cells expressing IL-33 (white arrows). (B) Smooth muscle actin in red and white lines delineated intestinal crypts. The white arrows point to SMA + IL-33 + cells which are located around vascular structures and crypts. (C) Some of the IL-33 + CD90 + cells are pointed by a white arrow. (D, E) CD45+IL-33+ cells in lamina propria , but not in epithelial compartment. (F) A few IL-33 + CD11c + where close to the epithelium of ACD patients. (G) Some of the CD20 + IL-33 + cells are pointed with white arrows. (H) Some of the lamina propria double positive IL-33 + CD64 + are pointed with white arrows. (I) EPCAM + IL-33 + cells are pointed with white arrows. The images were taken with 20X and 63X objectives from the SP5 Leica Microscope and objectives 20X, 40X and 60X from the Apotome Zeiss microscope.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Identification of dental pulp stem cells (DPSCs). Human DPSCs were positive for the cell surface antigens CD73, CD90, and CD105, as well as negative for CD14, CD20, CD34, and CD45 demonstrated by flow cytometry ( A ). DPSCs were cultured under osteogenic ( B , 14 days) or adipogenic ( C , 21 days) conditions, and showed mineralized nodules and lipid clusters as revealed by alizarin red and oil red staining, respectively. Scale bar = 400 ( B ) or 100 ( C ) mum.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 3 Allogeneic HCT in SHIV-C Infected, cART-treated RM. a Schematic of SHIV-C infection and allogeneic bone marrow transplantation strategy in SHIV-C infected, cART-treated RM. b Plasma Viral Loads (PVL) were measured longitudinally following transplant as specified in Fig. 1a . c White blood count (WBC) (x10 3 /uL). d Absolute Neutrophil Count (ANC) (x10 3 /uL). e Absolute Lymphocyte Count (ALC) (x10 3 /uL). f Platelet Count (PLT) (x10 3 /uL). g Hemoglobin (Hgb) concentration (g/dL). h Percent whole blood donor chimerism, measured by microsatellite analysis. i Percent donor myeloid (CD11b + CD3-) chimerism, measured flow cytometrically. j Percent donor CD4 + T cell (CD3+ CD4+ CD8- CD20- CD11b- lymphocytes) chimerism, measured flow cytometrically. k Percent donor CD8 + T cell (CD3+ CD8+ CD4- CD20- CD11b- lymphocytes) chimerism, measured by flow cytometry
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 1 Marker verification and validation of independent markers in BALL cells by immunofluorescence and flow cytometry. The expression of CD20 ( a - c ) and CD38 ( d - f ), were positive on BALL cell membranes. CD90f ( g - i ) and CD49f ( j , k ) were not expressed in BALL cells. DAPI indicates the cell nucleus. This finding was confirmed via the conducted flow cytometry analysis. Scale bar = 100 mum
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 11 Leukemia detection in nude mice after transplantation in the three SSC purification groups as assessed by blood flow cytometry and western blotting. A large number of BALL cells could be detected in the blood of recipient mice 2-3 weeks after transplantation in the density gradient centrifugation (DGU) group, but not in the blood of the flow cytometry sorting (FCMS) group and the immunomagnetic bead (MACS) group after 16 weeks of observation. CD20 detection in testicular tissue of nude mice after transplantation in the three groups by western blotting. High CD20 expression was detected in testicular tissue of nude mice in the density gradient centrifugation (DGU) group, but not in the flow cytometry sorting (FCMS) and immunomagnetic bead sorting (MACS) groups after 16 weeks of observation
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 5 Detection of testicular leukemia model mice. HE staining: a In normal C57BL/6 mice, a large number of spermatogenic cells and sperm cells could be seen in testis tissue. b In the model group, after 10 4 cells were injected to establish the model for 3 weeks, spermatogenic cells and sperm cells in the testis tissue decreased with the infiltration of BALL cells. Immunohistochemistry: c In normal C57BL/6 mice, the expression of CD20 protein in testicular tissue was negative. d In the model group, a large number of CD20-positive BALL cells infiltrated into the seminiferous tubule, spermatogenic cells and sperm cells in the testis tissue decreased. e Negative control. Scale bar = 100 mum
Conjugate: Biotin