Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 86-0209-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD20 Monoclonal Antibody (2H7), eVolve 655, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Notice: The eVolve line of antibody conjugates will be discontinued at the end of February, 2018 and replaced with our improved Invitrogen eBioscience Super Bright conjugated antibodies for flow cytometry. Individual eVovle catalog numbers will become unavailable as inventory depletes. This item has a corresponding Super Bright 645 format available please see Product # 64-0209-42. We will continue to offer a selection of Qdot conjugated antibodies. Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation. Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blod cells. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H7
- Vial size
- 25 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Anti-Human CD20 eVolve™ 655. Cells in the lymphocyte gate were used for analysis.