86-0209-41

antibody from Invitrogen Antibodies
Targeting: MS4A1 B1, Bp35, CD20, MS4A2
Provider product page for 86-0209-41

Antibody data

Product number
86-0209-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD20 Monoclonal Antibody (2H7), eVolve 655, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Notice: The eVolve™ line of antibody conjugates will be discontinued at the end of February, 2018 and replaced with our improved Invitrogen™ eBioscience™ Super Bright conjugated antibodies for flow cytometry. Individual eVovle catalog numbers will become unavailable as inventory depletes. This item has a corresponding Super Bright 645 format available – please see Product # 64-0209-42. We will continue to offer a selection of Qdot conjugated antibodies. Description: The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation. Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blod cells. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve™ 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
2H7
Vial size
25 Tests
Concentration
5 µl/Test
Storage
4° C, store in dark, DO NOT FREEZE!
MS4A1 protein structure - 86-0209-41 shown in red.