Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 86-0209-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD20 Monoclonal Antibody (2H7), eVolve 655, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The anti-human transferrin receptor antibody should be useful for the localization of transferrin receptors by immunofluorescence techniques. This antibody recognizes the human transferrin receptor and does not block binding of transferrin to the receptor. Although fluorescent transferrin conjugates have been widely used to study transferrin receptor-mediated endocytosis, antibodies to the transferrin receptor have proven to be useful for localizing transferrin receptors, studying endocytic recycling pathways and performing enzyme-linked immunosorbent assays (ELISAs). Upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2 mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H7
- Vial size
- 100 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Anti-Human CD20 eVolve™ 655. Cells in the lymphocyte gate were used for analysis.