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- Product number
- A03780-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD20/MS4A1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for CD20/MS4A1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse.
- Reactivity
- Mouse
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis.
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation.
Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway.
Qu Y, Yang H, Li S, Li L, Li Y, Wang D
International journal of biological macromolecules 2023 May 15;237:123927
International journal of biological macromolecules 2023 May 15;237:123927
Leflunomide Inhibits rat-to-Mouse Cardiac Xenograft Rejection by Suppressing Adaptive Immune Cell Response and NF-κB Signaling Activation.
Ma Y, Xie B, Guo J, Chen Y, Zhong M, Lin Q, Hua J, Zhong J, Luo X, Yan G, Dai H, Qi Z
Cell transplantation 2021 Jan-Dec;30:9636897211054503
Cell transplantation 2021 Jan-Dec;30:9636897211054503
Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway.
Yan YW, Fan J, Bai SL, Hou WJ, Li X, Tong H
PloS one 2016;11(2):e0148536
PloS one 2016;11(2):e0148536
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CD20/MS4A1 using anti-CD20/MS4A1 antibody (A03780-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD20/MS4A1 antigen affinity purified polyclonal antibody (Catalog # A03780-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD20/MS4A1 at approximately 33KD. The expected band size for CD20/MS4A1 is at 33KD.
- Additional image
