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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA1-37312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is tonsil tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 1 mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease.
Maeda S, Ohno K, Nakamura K, Uchida K, Nakashima K, Fukushima K, Tsukamoto A, Goto-Koshino Y, Fujino Y, Tsujimoto H
Veterinary journal (London, England : 1997) 2012 Oct;194(1):66-70
Veterinary journal (London, England : 1997) 2012 Oct;194(1):66-70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Raji Cells using anti-CD20 Polyclonal Antibody (Product # PA5-32313). The recommened dilution for this antibody in western blot applications is 1:25.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD20 Polyclonal Antibody (Product # PA5-32313) and a 33 kDa band corresponding to CD20 was observed in Raji, Ramos and Daudi. Membrane Enriched (40 µg lysate) of Raji (Lane 1), Ramos (Lane 2), Daudi (Lane 3), Jurkat (Lane 4), Hep G2 (Lane 5), Sp2/0-Ag14 (Lane 6), EL4 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CD20 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD20 Polyclonal Antibody (Product # PA5-32313) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane and cytoplasm localization. Panel e represents negative cell line Jurkat. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
