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- Antibody Data
- Antigen structure
- References [20]
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- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- NB600-1071 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1071, RRID:AB_10011605
- Product name
- Rat Monoclonal CD34 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Mouse, Rat
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4.
Prevention of perinatal nicotine-induced bone marrow mesenchymal stem cell myofibroblast differentiation by augmenting the lipofibroblast phenotype.
Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats.
Interleukin-17 potently increases non-small cell lung cancer growth.
Multivalent Forms of the Notch Ligand DLL-1 Enhance Antitumor T-cell Immunity in Lung Cancer and Improve Efficacy of EGFR-Targeted Therapy.
Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics.
Structure-based discovery of a small non-peptidic Neuropilins antagonist exerting in vitro and in vivo anti-tumor activity on breast cancer model.
Recombinant human acid sphingomyelinase as an adjuvant to sorafenib treatment of experimental liver cancer.
Myeloid hypoxia-inducible factor-1α is essential for skeletal muscle regeneration in mice.
The fractalkine/CX3CR1 system regulates β cell function and insulin secretion.
Endothelial cell HIF-1α and HIF-2α differentially regulate metastatic success.
The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing.
A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification.
Generation and characterization of a mouse lymphatic endothelial cell line.
Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells.
Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
Differential responsiveness to constitutive vs. inducible chemokines of immature and mature mouse dendritic cells.
Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
Kolb R, Kluz P, Tan ZW, Borcherding N, Bormann N, Vishwakarma A, Balcziak L, Zhu P, Davies BS, Gourronc F, Liu LZ, Ge X, Jiang BH, Gibson-Corley K, Klingelhutz A, Tan NS, Zhu Y, Sutterwala FS, Shen X, Zhang W
Oncogene 2019 Mar;38(13):2351-2363
Oncogene 2019 Mar;38(13):2351-2363
Prevention of perinatal nicotine-induced bone marrow mesenchymal stem cell myofibroblast differentiation by augmenting the lipofibroblast phenotype.
Sakurai R, Liu J, Wang Y, Torday JS, Rehan VK
Clinical science (London, England : 1979) 2018 Nov 15;132(21):2357-2368
Clinical science (London, England : 1979) 2018 Nov 15;132(21):2357-2368
Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats.
Xu B, Li Y, Deng B, Liu X, Wang L, Zhu QL
Experimental and therapeutic medicine 2017 Feb;13(2):588-594
Experimental and therapeutic medicine 2017 Feb;13(2):588-594
Interleukin-17 potently increases non-small cell lung cancer growth.
Wei L, Wang H, Yang F, Ding Q, Zhao J
Molecular medicine reports 2016 Feb;13(2):1673-80
Molecular medicine reports 2016 Feb;13(2):1673-80
Multivalent Forms of the Notch Ligand DLL-1 Enhance Antitumor T-cell Immunity in Lung Cancer and Improve Efficacy of EGFR-Targeted Therapy.
Biktasova AK, Dudimah DF, Uzhachenko RV, Park K, Akhter A, Arasada RR, Evans JV, Novitskiy SV, Tchekneva EE, Carbone DP, Shanker A, Dikov MM
Cancer research 2015 Nov 15;75(22):4728-41
Cancer research 2015 Nov 15;75(22):4728-41
Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics.
Huang YS, Li IH, Chueh SH, Hueng DY, Tai MC, Liang CM, Lien SB, Sytwu HK, Ma KH
Journal of tissue engineering and regenerative medicine 2015 Dec;9(12):E191-201
Journal of tissue engineering and regenerative medicine 2015 Dec;9(12):E191-201
Structure-based discovery of a small non-peptidic Neuropilins antagonist exerting in vitro and in vivo anti-tumor activity on breast cancer model.
Borriello L, Montès M, Lepelletier Y, Leforban B, Liu WQ, Demange L, Delhomme B, Pavoni S, Jarray R, Boucher JL, Dufour S, Hermine O, Garbay C, Hadj-Slimane R, Raynaud F
Cancer letters 2014 Jul 28;349(2):120-7
Cancer letters 2014 Jul 28;349(2):120-7
Recombinant human acid sphingomyelinase as an adjuvant to sorafenib treatment of experimental liver cancer.
Savić R, He X, Fiel I, Schuchman EH
PloS one 2013;8(5):e65620
PloS one 2013;8(5):e65620
Myeloid hypoxia-inducible factor-1α is essential for skeletal muscle regeneration in mice.
Scheerer N, Dehne N, Stockmann C, Swoboda S, Baba HA, Neugebauer A, Johnson RS, Fandrey J
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 1;191(1):407-14
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 1;191(1):407-14
The fractalkine/CX3CR1 system regulates β cell function and insulin secretion.
Lee YS, Morinaga H, Kim JJ, Lagakos W, Taylor S, Keshwani M, Perkins G, Dong H, Kayali AG, Sweet IR, Olefsky J
Cell 2013 Apr 11;153(2):413-25
Cell 2013 Apr 11;153(2):413-25
Endothelial cell HIF-1α and HIF-2α differentially regulate metastatic success.
Branco-Price C, Zhang N, Schnelle M, Evans C, Katschinski DM, Liao D, Ellies L, Johnson RS
Cancer cell 2012 Jan 17;21(1):52-65
Cancer cell 2012 Jan 17;21(1):52-65
The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing.
Fiorina P, Pietramaggiori G, Scherer SS, Jurewicz M, Mathews JC, Vergani A, Thomas G, Orsenigo E, Staudacher C, La Rosa S, Capella C, Carothers A, Zerwes HG, Luzi L, Abdi R, Orgill DP
Cell transplantation 2010;19(11):1369-81
Cell transplantation 2010;19(11):1369-81
A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification.
Kalabis J, Oyama K, Okawa T, Nakagawa H, Michaylira CZ, Stairs DB, Figueiredo JL, Mahmood U, Diehl JA, Herlyn M, Rustgi AK
The Journal of clinical investigation 2008 Dec;118(12):3860-9
The Journal of clinical investigation 2008 Dec;118(12):3860-9
Generation and characterization of a mouse lymphatic endothelial cell line.
Sironi M, Conti A, Bernasconi S, Fra AM, Pasqualini F, Nebuloni M, Lauri E, De Bortoli M, Mantovani A, Dejana E, Vecchi A
Cell and tissue research 2006 Jul;325(1):91-100
Cell and tissue research 2006 Jul;325(1):91-100
Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells.
Zheng Y, Du X, Wang W, Boucher M, Parimoo S, Stenn K
The Journal of investigative dermatology 2005 May;124(5):867-76
The Journal of investigative dermatology 2005 May;124(5):867-76
Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW
The Journal of investigative dermatology 2003 Apr;120(4):501-11
The Journal of investigative dermatology 2003 Apr;120(4):501-11
Differential responsiveness to constitutive vs. inducible chemokines of immature and mature mouse dendritic cells.
Vecchi A, Massimiliano L, Ramponi S, Luini W, Bernasconi S, Bonecchi R, Allavena P, Parmentier M, Mantovani A, Sozzani S
Journal of leukocyte biology 1999 Sep;66(3):489-94
Journal of leukocyte biology 1999 Sep;66(3):489-94
Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
Garlanda C, Berthier R, Garin J, Stoppacciaro A, Ruco L, Vittet D, Gulino D, Matteucci C, Mantovani A, Vecchi A, Dejana E
European journal of cell biology 1997 Aug;73(4):368-77
European journal of cell biology 1997 Aug;73(4):368-77
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A, Vecchi A
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants.
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A, Vecchi A
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
Arteriosclerosis, thrombosis, and vascular biology 1997 Aug;17(8):1599-604
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The staining was detected using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin. Note that this murine CD34 specific antibody stained only the endothelial cells of the tumor vasculature which is originating from the host animal cells (mouse). This observation was verified by including a negative control in this validation testing (see the IHC image of human renal cancer tissue section with negative signal for CD34).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin. Notably, the antibody specifically stained the endothelial cells of the tumor vasculature which is originating from the host animal cells (mouse). This observation was verified by including a negative control in this validation testing (see the IHC image of human renal cancer tissue section with negative signal for CD34).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the heart tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. The antibody specifically generated a staining of the endothelial cells in blood vessels of the colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which is an expected outcome for this clone. This section was included in NB600-1071's IHC validation testing as a negative control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor vasculature which are originating from the host (mouse).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
