Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PB9053 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD34 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, Direct ELISA in Human;Rat.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Exploring miR-21 as a key regulator in two distinct approaches of bone marrow stromal cells differentiation into Schwann-like cells.
Gualou Guizhi decoction promotes therapeutic angiogenesis via the miR210/HIF/VEGF pathway in vivo and in vitro.
Catalpol Prevents Glomerular Angiogenesis Induced by Advanced Glycation End Products via Inhibiting Galectin-3.
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties.
A mouse model of irradiation and spleen-thymus lymphocyte infusion induced aplastic anemia.
Novel dopamine-modified oxidized sodium alginate hydrogels promote angiogenesis and accelerate healing of chronic diabetic wounds.
The Effects of Inactive Platelet-rich Plasma at Different Injection Time on Prefabricated Flap Viability in Rabbits.
Construction of Urokinase-Type Plasminogen Activator Receptor-Targeted Heterostructures for Efficient Photothermal Chemotherapy against Cervical Cancer To Achieve Simultaneous Anticancer and Antiangiogenesis.
Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature.
Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia.
MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling.
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase.
Overexpression of inducible nitric oxide synthase impairs the survival of bone marrow stem cells transplanted into rat infarcted myocardium.
Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats.
Relationships of uPA and VEGF expression in esophageal cancer and microvascular density with tumorous invasion and metastasis.
Experimental study on inhibitory effects of diallyl sulfide on growth and invasion of human osteosarcoma MG-63 cells.
Liu YM, Wang HY, Wei CH, Li JP, Wang Y, Ma WZ, Jia H
Synapse (New York, N.Y.) 2024 May;78(3):e22293
Synapse (New York, N.Y.) 2024 May;78(3):e22293
Gualou Guizhi decoction promotes therapeutic angiogenesis via the miR210/HIF/VEGF pathway in vivo and in vitro.
Zhang Y, Cao Y, Li Y, Xiao L, Xu W, Xu W, Huang M, Zhang X, Chen Y, Nan L
Pharmaceutical biology 2023 Dec;61(1):779-789
Pharmaceutical biology 2023 Dec;61(1):779-789
Catalpol Prevents Glomerular Angiogenesis Induced by Advanced Glycation End Products via Inhibiting Galectin-3.
Sun WX, Gao YY, Cao Y, Lu JF, Lv GH, Xu HQ
Current medical science 2023 Aug;43(4):668-678
Current medical science 2023 Aug;43(4):668-678
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties.
Chen W, Xie G, Lu Y, Wang J, Feng B, Wang Q, Xu K, Bao J
Biomaterials 2022 Jan;280:121260
Biomaterials 2022 Jan;280:121260
A mouse model of irradiation and spleen-thymus lymphocyte infusion induced aplastic anemia.
Ni R, Fan L, Zhang L, Song Y, Wang H, Wang A, Liu B
Hematology (Amsterdam, Netherlands) 2022 Dec;27(1):932-945
Hematology (Amsterdam, Netherlands) 2022 Dec;27(1):932-945
Novel dopamine-modified oxidized sodium alginate hydrogels promote angiogenesis and accelerate healing of chronic diabetic wounds.
Chi J, Li A, Zou M, Wang S, Liu C, Hu R, Jiang Z, Liu W, Sun R, Han B
International journal of biological macromolecules 2022 Apr 1;203:492-504
International journal of biological macromolecules 2022 Apr 1;203:492-504
The Effects of Inactive Platelet-rich Plasma at Different Injection Time on Prefabricated Flap Viability in Rabbits.
Zhang H, Zhang T, Chen H, Zeng Y, Su Z, Fan J
Annals of plastic surgery 2021 Jun 1;86(6):701-706
Annals of plastic surgery 2021 Jun 1;86(6):701-706
Construction of Urokinase-Type Plasminogen Activator Receptor-Targeted Heterostructures for Efficient Photothermal Chemotherapy against Cervical Cancer To Achieve Simultaneous Anticancer and Antiangiogenesis.
Hu X, Mandika C, He L, You Y, Chang Y, Wang J, Chen T, Zhu X
ACS applied materials & interfaces 2019 Oct 30;11(43):39688-39705
ACS applied materials & interfaces 2019 Oct 30;11(43):39688-39705
Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature.
Zhou L, Liao Y, Wu J, Yang J, Zhang H, Wang X, Sun S
Therapeutics and clinical risk management 2018;14:1467-1481
Therapeutics and clinical risk management 2018;14:1467-1481
Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia.
Zhao Y, Zheng Y, Liu X, Luo Q, Wu D, Liu X, Zou L
Molecular human reproduction 2018 Mar 1;24(3):158-169
Molecular human reproduction 2018 Mar 1;24(3):158-169
MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling.
Guan H, Liu C, Fang F, Huang Y, Tao T, Ling Z, You Z, Han X, Chen S, Xu B, Chen M
Oncotarget 2017 Feb 28;8(9):14693-14707
Oncotarget 2017 Feb 28;8(9):14693-14707
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase.
Zhang Y, Chen Z, Wang T, Yang J, Li R, Wang S, Liu J, Ye Z
Oncotarget 2016 Jun 28;7(26):39302-39315
Oncotarget 2016 Jun 28;7(26):39302-39315
Overexpression of inducible nitric oxide synthase impairs the survival of bone marrow stem cells transplanted into rat infarcted myocardium.
Li HM, Liu L, Mei X, Chen H, Liu Z, Zhao X
Life sciences 2014 Jun 13;106(1-2):50-7
Life sciences 2014 Jun 13;106(1-2):50-7
Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats.
Tochinai R, Ando M, Suzuki T, Suzuki K, Nagata Y, Hata C, Uchida K, Kobayashi T, Kado S, Kaneko K
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2013 Sep;65(6):737-43
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2013 Sep;65(6):737-43
Relationships of uPA and VEGF expression in esophageal cancer and microvascular density with tumorous invasion and metastasis.
Jiang JT, Zhang LF, Zhou B, Zhang SQ, Li SM, Zhang W, Zhang J, Qiao Z, Kong RR, Ma YF, Chen S
Asian Pacific journal of cancer prevention : APJCP 2012;13(7):3379-83
Asian Pacific journal of cancer prevention : APJCP 2012;13(7):3379-83
Experimental study on inhibitory effects of diallyl sulfide on growth and invasion of human osteosarcoma MG-63 cells.
Hu Y, Chen L, Yi C, Yang F, Chen J
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2012 Aug;32(4):581-585
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2012 Aug;32(4):581-585
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
- Additional image